Essays about: "non-specific binding"

Showing result 1 - 5 of 10 essays containing the words non-specific binding.

  1. 1. Improving Caco-2 cell permeability assay using phospholipid covered silica beads

    University essay from Uppsala universitet/Institutionen för farmaci

    Author : Lana Faradj; [2021]
    Keywords : permeability; Caco-2; recovery; lipophilic; phosphatidylcholine; silica beads; peptide; plastic binding; bovine serum albumin;

    Abstract : The Caco-2 cell assay is widely used for in vitro permeability measurements. However, a draw back with the assay that this study will focus on improving, is compound adsorption to the plastic material. Lipophilic compounds such as Cyclosporin A and Peptide J, that will be used in this study, tend to bind to the plastic material in the assay. READ MORE

  3. 2. Electrical detection of proteins and nucleic acids using the electrokinetic effect in a silica microcapillary

    University essay from KTH/Skolan för informations- och kommunikationsteknik (ICT)

    Author : Kiarash Behnam; [2017]
    Keywords : ;

    Abstract : Electrical detection of humanized Immunoglobulin G and single stranded DNA having different mismatched contents is presented in this thesis. For electrical detection a simple method relying on streaming current measurement in a functionalized silica capillary was used. READ MORE

  4. 3. Development of a quantum dot based strategy for Gram-specific bacteria differentiation

    University essay from KTH/Skolan för teknik och hälsa (STH)

    Author : Ann-Lena Jahnsen; [2016]
    Keywords : Nanoparticles; Quantum dots; Bioconjugation; Diagnostics; Gram-specific; Bacteria detection;

    Abstract : Abstract Time-consuming diagnosis of bacterial blood stream infections and inappropriate antibiotic therapy have critical implications for patient outcome – with mortality figures rising for every hour of delayed treatment. The development of diagnostic methods that are capable of selective and rapid bacteria detection, and do not rely on preliminary blood culturing and Gram-staining procedures, is imperative in providing effective therapy and preventing multi-resistance. READ MORE

  5. 4. A generic capture assay for immunogenicity, using Biacore

    University essay from Institutionen för biologisk grundutbildning

    Author : Martin Engqvist; [2013]
    Keywords : Immunogenicity; immunoassay; capture assay; Biacore; non-specific binding; anti-drug antibodies ADA ; human plasma; anti-biotin; biotinylation;

    Abstract : The purpose of this investigation was to create and optimise a capture assay for the detectionof anti-drug antibodies (ADA) in human plasma, using Biacore. We also dealt with the nonspecificplasma binding to mouse-derived anti-biotin which may occur in the capture assay. READ MORE

  6. 5. Weak Affinity Chromatography : Evaluation of Different Silica Supports for Protein Immobilizationand Effect of Mobile Phases Regarding Retentionand Non-specific Binding

    University essay from Institutionen för naturvetenskap, NV

    Author : David Westerhult; [2012]
    Keywords : ;

    Abstract : Fragment based lead discovery (FBLD), where libraries of small fragments are screened andlater on developed to lead compounds, is an alternative to the classical drug discovery methods such as high trough-put screening. Weak affinity chromatography (WAC) is a new promising approach to the screening process of FBLD. READ MORE