Unfolding probes : A novel method for biomolecular detection

Abstract: Unfolding probes is a novel detection method for biomolecules, involving an oligonucleotide probe that can be amplified and which thereby gives rise to a strong signal for individual probe - target interactions. The present project focuses on a variant of unfolding probes called 2-fold probes and on optimizing the detection efficiency of synthetic targets. Furthermore, proof of concept experiments were performed for the detection of Her2 mRNA in cells.