Selective Quantification of Co-Eluting Proteins by Means of Partial Least Squares

Abstract: The aim of this thesis is to create a model of the correlation between absorbance and concentration of co-eluting proteins. A separation using the chromatograph system ÄKTA from GE-Health care of BSA,Lysozyme and IgG was performed. Absorbance was measured at three different wave lengths. Since the mentioned proteins do not co-elute in a neutral pH the experiments were performed at a high pH-level which rendered the experiments unsuccessful. A PLS-function was written in Matlab. The attempt to create a model of the correlation was found futile since the data obtained from experiments did not contain enough information about the proteins. The written PLS-model was evaluated using an old data set and was found to be a useful and fast algorithm