Detection and Quantification of PCB insoil using GC/MS : - method development and education for users

University essay from KTH/Skolan för teknikvetenskap (SCI)

Author: Elias Saba; [2013]

Keywords: Transformative learning; GC; MS; PCB; teaching; pedagogics; detection; quantification; quadrupole MS; internal standard; chemical analysis; user manual; artefact; cognitivism; social constructivism.; Transformativ lärande; GC; MS; PCB; lärande; pedagogik; detektion; kvantifiering; kvadrupol MS; intern standard; kemisk analys; användamanual; artefakt; kognitivism; social konstruktivism.;

Abstract: The aim of this paper is to document the development of a method for detectionand quantification of Polychlorinated biphenyls (PCB) in soil using GasChromatography (GC) connected to a Quadrupole Mass Spectrometer (MS) via aninternal standard (CB189). The method developed is performed in conjunction withthe information provided by the Swedish environmental agency (Svenskanaturvårdsverket, SNV) in regards to PCB limits for sensitive land usage. The steps ofthe method and maintenance of the GC/MS are used to create a user manual andan attempt at transformative learning is done in an effort to teach the staff atLjungaLab AB so that at the very least, independent analysis can be run and at best,new methods and application are independently developed for the GC/MS. Anevaluation of the teaching efforts is also done to assess what grade of learning isachieved.

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