Multipoint spectroscopic analyzing & imaging method

Abstract: Spectroscopy is a technique as the interaction of different radiation spectrum with matter to analysis of a sample. This thesis work proposed two methods are multiple pointes spectroscopies analyzing then imaging detection methods for solid samples. Developed method one is using Ultraviolet (UV), Visible (Vis) and Infrared (IR) detection. Where detection was assembled with deuterium as well tungsten-halogen lamp source (which were able to generate 175 nm to 3300 nm wavelength), a manual X-Y stepper for scan an inhomogeneous biological sample, optical design beside Indium gallium arsenide (InGaAs) detection unit was used of Lamda 950 by PerkingElmer. Second improved methodology is Vis detection imaging of samples. In Vis detection imaging was constructed with Helium-Neon (HeNe) red laser as a source (able to generate 632.8 nm wavelengths), a silicon pin photodiode detector, lens, multimeter, X-Y positioner stepper motors to scan samples. The work show successfully detected and imaged of water, fresh leaf, brain phantom in addition 3mm horizontal and 1.5 mm vertical cooper line. The thesis works proposed methods has obtained accurate results of all the samples detection specifically has devised imaging of samples. This spectroscopic process is suitable for any type of liquid, solid also gas detecting moreover imaging approach can be applicable in any type of inhomogeneous matter.