Essays about: "5´ UTR"

Found 5 essays containing the words 5´ UTR.

1. 1. Increased expression of therapeutic proteins by identification of 3'-UTRs from high expressing genes in CHO cells

   University essay from Linköpings universitet/Institutionen för fysik, kemi och biologi

   Author : Alexander Westlund; [2019]
   Keywords : Mammalian cell; CHO; E. coli; protein expression; plasmid; transformation; Fc-fusion protein; flow cytometry; FACS; transfection; mRNA; UTR; gene engineering;

   Abstract : Therapeutic proteins, a.k.a. biopharmaceuticals, are most commonly produced in expression systems derived from Chinese Hamstery Ovary (CHO) cells, thanks to great capacity of post-translational modifications like secretation, folding and glycosylation. READ MORE

3. 2. Method for tracking orthogonal ribosomes in vivo using MS2coat protein

   University essay from Uppsala universitet/Molekylärbiologi

   Author : John Lindström; [2019]
   Keywords : ;

   Abstract : Ribosomes are large macromolecules responsible for protein synthesisand they consist of both RNA and proteins. Each ribosome is made of one large and one small subunit. Even though the ribosome is one of the most studied machineries in the cell there is a gap in our understanding of how this macromolecule functions in vivo. READ MORE

4. 3. Evaluation of 5´- and 3´-UTR Translation Enhancing Sequences to Improve Translation of Proteins in CHO Cells

   University essay from Linköpings universitet/Teknisk biologi

   Author : Ellen Einarsson; [2018]
   Keywords : Regulation; Untranslated regions; 5´ UTR; 3´ UTR; mRNA; Expression; Flow cytometry;

   Abstract : The purpose of this project was to identify and evaluate nucleotide sequences enhancing translation of proteins in Chinese hamster ovary (CHO) cells. Candidate sequences were placed in the 5´-untranslated region (UTR) or 3´ UTR respectively and evaluated in a CHO-based expression system with a fluorescent Fc-fusion protein as a model protein. READ MORE

5. 4. Analysis of conserved microRNA targets in the nematode Caenorhabditis elegans and the fruit fly Drosophila melanogaster.

   University essay from Institutionen för naturvetenskap, miljö och teknik

   Author : Ninwa Youssef; [2013]
   Keywords : ;

   Abstract : MicroRNA (miRNA) is small regulatory non-coding single stranded RNA molecule that can repress protein expression either at transcriptional or translational level. Since their discoveries in nematodes in the early 1990´s extensive research have shown that this mechanism is conserved across species. READ MORE

6. 5. Mutational analysis of the csgD mRNA leader: search for a mode of regulation

   University essay from Institutionen för biologisk grundutbildning

   Author : Linnea Jonsäll; [2013]
   Keywords : Regulatory small RNA; enterobacteria; CsgD; OmrA; OmrB; mutational analysis; in vivo and in vitro translation assay;

   Abstract : The CsgD protein is the master regulator of a pathway leading to the formation of curli, in essence regulating the switch between a motile and a sessile lifestyle for bacteria. The 5’-UTR region of the csgD mRNA is a hotspot for multiple regulatory small RNAs (sRNA) involved in a complex regulatory network. READ MORE