Essays about: "Actinobacteria"

Found 4 essays containing the word Actinobacteria.

  1. 1. Purification, functional characterization and crystallization of the PerR peroxide sensor from Saccharopolyspora erythraea

    University essay from Uppsala universitet/Strukturbiologi

    Author : Grim Elison Kalman; [2019]
    Keywords : structural biology; cell culture; protein expression; genetic engineering; spectroscopy; X-ray crystallography; small angle X-ray scattering; SAXS; transcription factor; PerR; metal binding; antibiotics; Saccharopolyspora erythraea; S. erythraea; soil bacterium; actinobacteria; actinomycetes; oxidative stress; peroxide stress; structure determination; characterization; stabilization; peroxide sensor; iron sensor; manganese sensor; metalloprotein; metal ion homeostasis; metal ion; prokaryotic; strukturbiologi; cellodling; proteinuttryck; genteknik; spektroskopi; röntgenkristallografi; småvinkel-röntgenspridning; SAXS; transkriptionsfaktor; PerR; metallbindande; antibiotika; Saccharopolyspora erythraea; S. erythraea; jordartsbakterie; Aktinobakterier; Actinobacteria; Actinomycetes; oxidativ stress; peroxid stress; strukturbestämning; karaktärisering; stabilisering; peroxidsensor; järnsensor; mangansensor; metalljonshomeostas; metalljoner; prokaryot;

    Abstract : This report summarizes the work on the cloning, expression, and purification of PerR, a metal sensing regulator from Saccharopolyspora erythraea and the subsequent characterization using small angle X-ray scattering and other biochemical methods. The report aims to provide an insight into prokaryotic metal homeostasis, provide a better understanding of how PerR works and provide valuable information for the continued work on the crystallization of PerR. READ MORE

  2. 2. HisA mutants with minor structural differences display major functional deviations

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildningUppsala universitet/Struktur- och molekylärbiologi

    Author : András Erdélyi; [2016]
    Keywords : ;

    Abstract : Even though enzymes tend to specialize on one reaction during evolution, enzyme promiscuity is an abundant phenomenon. The subject of this thesis is the Salmonella enterica N’-[(5’-phosphosoribosyl)-formimino]-5-aminoimidazole-4 carboxamide-ribonucleotide (ProFAR) isomerase (SeHisA), a (βα)8-barrel enzyme from the histidine biosynthesis that catalyzes one reaction on one substrate in one organism. READ MORE

  3. 3. Development of PCR primers for community studies of bacterial hydrogenases in environmental samples

    University essay from SLU/Dept. of Plant Biology and Forest Genetics (until 131231)

    Author : Ernest Ndobe Ndobe; [2013]
    Keywords : uptake hydrogenase; bacterial community; biological nitrogen fixation; symbiosis;

    Abstract : Soil microorganisms interact with plants in ways that affect nutrient availability, hormonal regulation and often lead to improved plant growth. In the N2-fixing root nodule symbiosis between legume plants and bacteria generally called rhizobia the plant gains access to atmospheric nitrogen. READ MORE

  4. 4. Microbial Diversity in Raw and Pasteurized Milk with Terminal Restriction Fragment Length Polymorphism (T-RFLP)

    University essay from Lunds universitet/Examensarbeten i molekylärbiologi

    Author : Sofia Svenningsson; [2012]
    Keywords : Biology and Life Sciences;

    Abstract : Abstract In this thesis, a molecular PCR-based method was used to study the bacterial diversity in milk. The aim was to compare the microbiota of traditionally pasteurized milk with milk treated with a novel pasteurization technique, using terminal restriction fragment length polymorphism (T-RFLP). READ MORE