Essays about: "Anders Kristoffersson"

Found 1 essay containing the words Anders Kristoffersson.

  1. 1. Rolling circle transcription on smallest size double stranded DNA minicircles

    University essay from Institutionen för biologisk grundutbildning

    Author : Anders Kristoffersson; [2010]
    Keywords : RNA polymerase; RNAP T7; DNA nanotechnology; molecular motor; real time monitoring of transcription; 2’ O methyl RNA; molecular beacon; minicircle;

    Abstract : The RNA polymerase T7 is utilized as a component of motor complexes in DNA nanotechnology due to its high promotor specificity, the lack of external transcription factors and its very high processivity, but there is no experience of its application on small double stranded DNA circles. Circular templates from 210 to 126 bp in circumference sharing a common promotor termination motif were synthesized and transcription was monitored at end point on gel and in real time with a 2’ O methyl RNA molecular beacon. READ MORE