Essays about: "BL21 DE3"
Showing result 1 - 5 of 13 essays containing the words BL21 DE3.
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1. Recombinant expression and purification of sugar beet Phytoglobins in Escherichia Coli BL21 (DE3) including metabolic engineering of the heme biosynthesis
University essay from Lunds universitet/Tillämpad biokemi; Lunds universitet/BeräkningskemiAbstract : The protein hemoglobin (Hb) is vital for us humans to ensure enough oxygen replacement in the body. Hb-resembling proteins also exist in plants, these are called phytoglobins (Pgbs). Pgbs are very interesting since they can play a considerable role in the development of blood substitutes and iron supplements for human use. READ MORE
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2. Production of 5-ketogluconic acid using recombinant strains of Gluconobacter oxydans
University essay from Lunds universitet/Bioteknik (CI); Lunds universitet/Bioteknik (master)Abstract : 5-Keto-D-gluconic acid (5-KGA) is a valuable compound that has a wide range of applications in the chemical and food industries and is commonly used as a precursor for manufacturing tartaric acid (TA). Industrial production of 5-KGA through chemical approaches was practiced in the early 1920’s and was dropped immediately due to several drawbacks because of its complicated process that resulted in lower 5-KGA yield and higher accumulation of impure gluconic acid. READ MORE
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3. Understanding the Involvement of Leukocyte Cell-derived Chemotaxin 2 (LECT2) in Amyloidosis
University essay from Linköpings universitet/Institutionen för fysik, kemi och biologiAbstract : Leukocyte cell-derived chemotaxin 2 (LECT2) is a zinc-binding multi-functional protein comprising three disulfide bonds, that is involved in multiple disorders of worldwide concern. Recently LECT2 was found to be involved in amyloidosis (ALECT2) and is believed to be the third most common form of systemic amyloidosis. READ MORE
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4. Production of gangliosidebiosynthetic membrane enzymes for biochemical and functionalstudies : Expression, purification and crystallizationoptimization of Thermococcus onnurineus Dolicho l-phosphate mannose synthase, Homosapiens and Branchiostoma floridae Glucosylceramide synthase
University essay from KTH/Skolan för kemi, bioteknologi och hälsa (CBH)Abstract : Glycolipids play important roles in the biology of prokaryotes and eukaryotes, including humans, and although theyare found on the cell-membrane surface of all eukaryotic cells, not much is known about their biosynthesis. The aim ofthis project was to characterize two enzymes: glucosylceramide synthase (GCS) which is involved in the biosynthesisof glycolipids such as gangliosides that are abundant in the membranes of nerve cells; and dolicholphosphate mannosesynthase (DPMS), involved in the synthesis precursor for protein glycosylation. READ MORE
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5. Cloning, expression and production of Vicia faba Leghemoglobin B
University essay from Lunds universitet/Tillämpad biokemiAbstract : In this thesis a preliminary evaluation of the potential of a leghemoglobin (Lb) for the development of a blood substitute in humans, resembling hemoglobin (Hb) in red blood cells, was conducted. Leghemoglobin B (VfLbB), a gene from Vicia faba, was cloned into a pET-DEST42 vector, using the Gateway™ recombination technology. READ MORE
