Essays about: "CRISPR Cas9"

Showing result 1 - 5 of 40 essays containing the words CRISPR Cas9.

  1. 1. Evaluation of strategies for construction of protease libraries in yeast.

    University essay from Lunds universitet/Teknisk mikrobiologi; Lunds universitet/Bioteknik

    Author : Sidharth Jaya Sankar; [2022]
    Keywords : Yeast; GFP-fusion technology; protease; applied microbiology; Technology and Engineering;

    Abstract : Proteases are a group of enzymes that are involved in breaking down larger proteins into smaller polypeptides or single amino acids, and they are used in various applications. In this project, the potential of budding yeast Saccharomyces cerevisiae as host for the construction of protease libraries is evaluated. READ MORE

  2. 2. Generation of induced pluripotent stem cells (iPSCs) lines deficient for genes associated with neurodevelopmental diseases using CRISPR/Cas9 technology

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Claudia De Guidi; [2021]
    Keywords : ;

    Abstract : Induced pluripotent stem cells (iPSCs) can self-renew and differentiate into many other cell types. IPSCs are derived from somatic cells, and upon reprogramming, they share an expression profile similar to embryonic stem cells (ESCs). READ MORE

  3. 3. Potential Application of Multiplex Automated Genome Engineering (MAGE) and One-Step Curing Plasmid System for Environmental Cambodian Enterobacterial Isolates

    University essay from Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

    Author : Olivia Alexandra; [2021]
    Keywords : Antimicrobial resistance; plasmid curing; environmental isolates; recombineering; enterobacteria;

    Abstract : Antimicrobial resistance (AMR) is concerning because it limits antimicrobial drug treatment options. AMR occurs by the overuse and misuse of antimicrobial drugs. In environmental settings, AMR can disseminate from places of high use, which leads to increased exposure to humans and animals. READ MORE

  4. 4. The use of a CRISPR-Cas9 system to protect probiotic strains from transferrable drug resistance genes

    University essay from Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

    Author : Sara Lundberg; [2021]
    Keywords : CRISPR-Cas9; antibiotic resistance; horizontal gene transfer; probiotic bacteria;

    Abstract : The discovery of antibiotics have revolutionized modern medicine, facilitating the treatment of a variety of bacterial infections, enabled surgeries otherwise impossible to perform and increased life expectancy in all countries. However, the rapid development of resistance among microorganisms and the increasing numbers of clinical outbreaks caused by multiresistant bacteria have accelerated the need for new alternatives to antibiotics. READ MORE

  5. 5. Visualizing neuronal cell sub-populations using novel transgenic zebrafish lines.

    University essay from Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

    Author : Aikaterini Zafeiriou; [2021]
    Keywords : Zebrafish; CRISPR Cas9; locomotion; confocal microscopy; immunohistochemistry;

    Abstract : Zebrafish is a frequently used model organism with an array of transgenic lines that have been used indevelopmental and physiological studies. We aim to generate novel transgenic zebrafish reporter lines to study subpopulations of spinal neurons in vivo. READ MORE