Essays about: "Calcium-dependent"

Found 4 essays containing the word Calcium-dependent.

  1. 1. Characterization of novel ZCa-based variants to investigate calcium dependent target interaction

    University essay from KTH/Proteinvetenskap

    Author : Gabriella Rossi; [2021]
    Keywords : ZCa; IgE; IL-23; recycling antibody; calcium-dependency;

    Abstract : ZCa is an engineered three-helical affinity protein that through replacement of the loop between helix two and three with an EF-hand motif has a calcium-dependent binding to its target. By using the difference of calcium concentration in the plasma and the cell along with the favorable properties of ZCa, the interest in creating therapeutics toward different diseases based on the ZCa scaffold has come about. READ MORE

  3. 2. Optimization of Calcium-Dependent Affinity Ligands for Protein Purification

    University essay from KTH/Proteinvetenskap

    Author : Linnea Öst; [2021]
    Keywords : Protein purification; Protein A; calcium-dependent; scFv; G-CSF; multimerization; mild elution; ZCa;

    Abstract : With an expanding life-science sector and growing production of recombinant proteins, the need for efficient downstream processing is increasing. Certain proteins are sensitive to the harsh conditions often used in protein purification, such as low pH, which can result in aggregation and denaturation. READ MORE

  4. 3. Calcium-dependent affinity ligands for protein purification

    University essay from KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

    Author : Emma Larsson; [2020]
    Keywords : Purification; Affinity chromatography; Protein A; Z domain; Calcium-dependent; ZCa; Elution;

    Abstract : The rapid growth of the biopharmaceutical industry has led to increasing demands on the protein production process. An important aspect is the yield of functional protein, which can be greatly affected by the choice of downstream purification. READ MORE

  5. 4. Selection of a calcium-dependent IgG1-binding protein domain

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Sanne Rönning; [2020]
    Keywords : protein science; phage display; selection; Protein A; chromatography; calcium dependence; characterization; proteinteknik; fagdisplay; selektion; Protein A; kromatografi; calciumberoende; karakterisering;

    Abstract : Standard purification processes in large scale antibody production are largely dependent on Protein A chromatography. Protein A binds specifically to many subclasses of IgG with high affinity. However, in order to elute the proteins, the pH needs to be lowered. READ MORE