Essays about: "Deglycosylation"

Found 2 essays containing the word Deglycosylation.

  1. 1. Structure-function study on the activation mechanism of Endo T : Crystallization and biochemical characterization of the intact Endo T from Trichoderma reesei (Hypocrea jecorina)

    University essay from SLU/Dept. of Molecular Biology (until 131231)

    Author : Jonathan Boel; [2011]
    Keywords : Hypocrea jecorina Endo T; glycoside hydrolase family 18; EC 3.2.1.96; deglycosylation; proteolysis; C-terminus; protein structure;

    Abstract : Endo T is a deglycosylating enzyme secreted by the filamentous fungus Trichoderma reesei (Hypocrea jecorina). The active Endo T protein is both deglycosylated and C-terminal processed. Since the intact protein was never observed in the T. reesei culture medium, a construct of the intact Endo T was expressed in P. READ MORE

  3. 2. Mutational engineering, growth selection, expression, purification and crystallization of ligand bound endo-beta-N-acetylglucosaminidase T (Endo-T) from Hypocrea jecorina

    University essay from SLU/Dept. of Molecular Biology (until 131231)

    Author : Andreas Digre; [2010]
    Keywords : Endo-T; Endo-beta-N-acetylglucosaminidase; Deglycosylation; EC 3.2.1.96; Crystallography; Ligand bound structure; High mannose N-glycan; HPAEC-PAD;

    Abstract : Recently, one of the first fungal-expressed, deglycosylating, Endo-beta-N-acetylglucosaminidases was found in the extracellular medium of soft-rot ascomycete Hypocrea jecorina (a.k.a. Trichoderma reesei) belonging to glycoside hydrolase family 18 (GH18). READ MORE