Essays about: "Dictyostelium discoideum"

Found 5 essays containing the words Dictyostelium discoideum.

  1. 1. Expression of FLAG-tagged argonautes in Dictyostelium discoideum

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning; Uppsala universitet/Institutionen för cell- och molekylärbiologi

    Author : Zozek Abdul Rahman; [2022]
    Keywords : Dictyostelium; discoideum; Argonaute; Protein; FLAG; FLAG-tag; Molecular Biology; Microbiology; SorMC; agnA; agnC; agnE; electrophoresis; pDM304;

    Abstract : Argonautes are conserved RNA-binding proteins that can regulate gene expression post transcriptionally through a process known as RNA interference (RNAi). This is done through the use of small RNAs, e.g. sRNAs that act as a guide for the argonautes, allowing for sequence-specific binding to the target site. READ MORE

  3. 2. Search for the Argonaute protein that governs miRNA regulation in Dictyostelium discoideum

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Miranda Åström; [2021]
    Keywords : Argonaute proteins; RNA interference; miRNA; 3xFLAG-tag;

    Abstract : MicroRNAs are small non-coding RNAs that regulate gene expression through RNA interference. These small RNAs enact gene silencing by forming a RNA-inducing silencing complex together with the effector protein Argonaute. The function of the Argonautes in the social amoeba Dictyostelium discoideum is not yet fully understood. READ MORE

  4. 3. Host-pathogen interactions using Dictyostelium discoideum as host model

    University essay from SLU/Dept. of Molecular Biology (until 131231)

    Author : Ida Lindström; [2011]
    Keywords : Dictyostelium discoideum; Salmonella typhimurium; non-coding RNAs; infection;

    Abstract : Dictyostelium discoideum has long been used as a model organism to study many different biological processes. The amoeba is very similar to macrophages present in higher animals, for example humans. Furthermore, several genes in D. discoideum have shown to be homologous with human genes. READ MORE

  5. 4. The role and genetic control of non-coding RNAs in bacterial infection

    University essay from SLU/Dept. of Molecular Biology (until 131231)

    Author : Jonas Åkerblom; [2010]
    Keywords : Dictyostelium discoideum; non-coding RNA; RNAi; Legionella pneumophila; bacterial infection; miRNA; siRNA; promoter;

    Abstract : Promoters are genetic elements that facilitate the transcription of a gene and they have been found in front of non-coding RNA (ncRNA) genes in different organ-isms, e.g. the model plant Arabidopsis thaliana. READ MORE

  6. 5. mRNA and natural endogenous antisense RNA in Dictyostelium discoideum

    University essay from SLU/Dept. of Molecular Biosciences

    Author : Louise Andersson; [2006]
    Keywords : RNAi; mRNA; antisense RNA; Dictyostelium discoideum;

    Abstract : The aim of this Master's Thesis was to investigate the presence and regulation of RNAi in the model organism Dictyostelium discoideum. The background for the project is a cDNA library representing 18-25nt RNAs (9000 clones) from Dictyostelium discoideum that recently was constructed. READ MORE