Essays about: "EGFP"

Showing result 1 - 5 of 12 essays containing the word EGFP.

  1. 1. 3′-end fluorescence labelling of long RNAs using terminal deoxynucleotidyl transferase

    University essay from Lunds universitet/Kemiska institutionen

    Author : Johannes Lidberg; [2022]
    Keywords : Physical biochemistry; Fluorescence; In vitro transcription; RNA; Terminal deoxynucleotidyl transferase; Nucleobase analogue; Chemistry;

    Abstract : Through recent years, RNA has rapidly gone from mainly being known as a passive information-carrying molecule within the central dogma of molecular biology to becoming the active ingredient in multiple therapeutics, such as in vaccines and potential drugs against cancer. However, one of the main challenges with RNA as a therapeutic is efficient delivery to cells. READ MORE

  3. 2. The use of Gibson Assembly for DNA cloning

    University essay from KTH/Tillämpad fysik

    Author : Samuel Johansson; [2022]
    Keywords : Plasmid; Primer; Cloning; PCR; Gibson-Assembly; Transfection; Fluorescent protein; Fluorescent imaging; mCherry; EGFP; Zona-Occludens 1; Occludin; alphaTubulin; Cx-43-7; Plasmid; Primer; Kloning; PCR; Gibson-Assembly; Transfektion; Fluorescerande protein; Fluorescerande avbildning; mCherry; EGFP; Zona-Occludens 1; Occludin; alphaTubulin; Cx-43-7;

    Abstract : This thesis report revolved around the cloning process of plasmids. Attempts of cloning the red fluorescent protein mCherry, and the green fluorescent protein EGFP from various plasmids, into other plasmids containing different cell-junction/cytoskeleton plasmids were made. READ MORE

  4. 3. Hypoxia-induced expression of HIF-1a and GPR30, in relation to survival, proliferation and apoptosis in MCF-7 and SkBr3 breast cancer cells.

    University essay from Lunds universitet/Läkarutbildningen

    Author : Hanna Chahin; Dianna Jazera; [2021]
    Keywords : hypoxia; breast cancer; GPR30 estrogen receptor; Medicine and Health Sciences;

    Abstract : Introduction: Hypoxia that accompanies tumor growth induces the expression of hypoxiainducible factor a (HIF-1a), in turn mediating the expression of other genes, including G protein-coupled receptor 30 (GPR30), to promote adaption to the hypoxic microenvironment and enable cell proliferation and survival. Nonetheless, this correlation has not been causally explained. READ MORE

  5. 4. Improving the temporal resolution of a microspectrometer for the study of the photophysics of enhanced green fluorescent protein

    University essay from KTH/Tillämpad fysik

    Author : Lukas Rane; [2021]
    Keywords : Biophysics; EGFP; Fluorescence; triplet state; Biofysik; EGFP; fluorescens; triplet tillstånd;

    Abstract : The use of fluorescent proteins as fluorescent markers has exploded over the last decades. In particular due to the development of advanced microscopy for live cell measurements, dynamic molecular studies down to single molecule levels and for superresolution microscopy. READ MORE

  6. 5. Effects of a phthalate mixture on Wnt/β-catenin signaling, apoptosis and metabolic rate in zebrafish embryos

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Stavros Bountis; [2020]
    Keywords : phthalates; Wnt β-catenin signaling; apoptosis; zebrafish; metabolic rate;

    Abstract : One of the most common plasticizers used in the production of plastic are phthalates. These chemicals have been associated with many adverse effects including developmental and reproductive anomalies. Early developmental processes targeted by chemicals can have long-lasting effects on individuals. READ MORE