Essays about: "Glycosylation"

Showing result 1 - 5 of 18 essays containing the word Glycosylation.

  1. 1. Performance of SpheriCal® standards as calibrants for IgG glycopeptide analysis using MALDI-MS

    University essay from KTH/Tillämpad fysikalisk kemi

    Author : Sandra Bubic; Martin Kjellberg; Ludvig Samuelsson; [2022]
    Keywords : biomarker; MALDI-MS; IgG; glycosylation; calibration; SpheriCal®; biomarkör; MALDI-MS; IgG; glykosylering; kalibrering; SpheriCal®;

    Abstract : I dagens samhälle finns alla möjliga sorters sjukdomar - alltifrån den vanliga säsongsinfluensan till mer allvarliga infektioner och kroniska sjukdomar som cancer och Alzheimers. Därför finns även ett stort intresse för att kunna ställa rätt diagnos samt söka möjliga behandlingar och att bota dessa sjukdomar. READ MORE

  3. 2. Hello P. pastoris! : The cultivatin and expression of proteins in the yeast Pichia pastoris

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Håkansson Anja; Dalén Therese; Gröblacher Josefine; Göransson William; Jaksties Jonathan; Ortstad Nathalie; Lenkeit Gesser Pauline; [2022]
    Keywords : Affibody AB; Pichia pastoris; pAOX1; pAOX2; pFLD1; pGAP; cultivation; fermentation; glycosylation;

    Abstract : Producing pharmaceuticals in Escherichia coli inevitably comes with an extensive purification process. This is because many of the native proteins of E. coli are immunogenic to humans, especially the heat and pH resistant endotoxins located in the membrane of E. coli. READ MORE

  4. 3. Comparison of Lectins and their suitability in Lectin Affinity Chromatography for isolation of Glycoproteins

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Pontus Andersson; [2020]
    Keywords : biotechnology; lectins; chromatography; glycoproteins; lectin affinity chromatography; LAC; ConA; Concanavalin A; bioteknik; lektiner; kromatografi; affinitetskromatografi; glykoproteiner; ConA; Concanavalin A;

    Abstract : Virtually all extracellular proteins in humans are glycoproteins and likewise are many biopharmaceuticals. The glycosylation is directly correlated to biological function and stability of these proteins. The ability to isolate glycoproteins is thus of great importance in many applications. READ MORE

  5. 4. Development of an analysis method for a glycosylated protein using MALDI-MS and separation techniques

    University essay from KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

    Author : Jessica Singh; [2020]
    Keywords : Immunoglobulin G; glycopeptide enrichment; Glycans; MALDI; CE-MS;

    Abstract : The antibody Immunoglobulin G (IgG) main function is to protect and prevent the body from infections, and it is normally found in human serum. This study is about IgG glycosylation, which is associated with different types of diseases such as neurological diseases, cancers and immunodeficiency etc. READ MORE

  6. 5. Optimizing signal peptides for expression of recombinant antibodies in HEK293 cells

    University essay from Linköpings universitet/Institutionen för fysik, kemi och biologi

    Author : Gustav Myhrinder; [2020]
    Keywords : Signal peptide; antibody; mammalian cell; HEK293; secretory pathway;

    Abstract : Monoclonal antibodies are well-established as a therapeutic in the biopharmaceutical market, targeting a variety of diseases and with 79 approved products by the United States Food and Drug Administration in December 2019. Therapeutic monoclonal antibodies are commonly produced as recombinant proteins in mammalian cell lines, due to their capacity of post-translational modifications, most notably glycosylation. READ MORE