Essays about: "Gradient centrifugation"

Showing result 1 - 5 of 11 essays containing the words Gradient centrifugation.

1. 1. Microfluidic sorting of sperm for assisted reproduction - An evaluation of ZyMōt multi sperm separation device

   University essay from Göteborgs universitet / Instiutionen för biologi och miljövetenskap

   Author : Emma Olofsson; [2023-06-22]
   Keywords : Assisted reproduction technologies Spermatozoa DNA fragmentation Density-gra- dient centrifugation Microfluidics ZyMōt Multi;

   Abstract : Infertility is a worldwide problem and the main reason for the use of assisted reproductive technol- ogy (ART). In-vitro fertilization (IVF) is one ART treatment normally used, where the fertilization of oocytes occurs in vitro. READ MORE

3. 2. Design, expression and purification of virus-like particles derived from metagenomic studies : Virus-like Particles (VLP) of novel Partitiviridae species, Hubei.PLV 11, and novel Soutern pygmy squid flavilike virus were designed, expressed using the bac-to-bac expression system and then pruified using various methods

   University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

   Author : Diyar Ayranci; [2021]
   Keywords : Virus; virus-like particles; VLP. Western Blot; SDS-PAGE; Chromatography; partitivrus; partitiviridae; flaviviridae; flavivirus; Hubei partitilike virus; Southern Pygmy Squid flavilike virus; protein purification; protein characterization; PCR; Cloning; metagenomic; strucutral biology; cryo-em; bac-to-bac expression system; vaccines; arbovirus; arthropod-borne virus;

   Abstract : Viruses are entities which are made of a few genes and are reliant on obligate parasitism to propagate. Due to the obligate connection to their hosts, virus evolution is constrained to the type of host. Viruses however do transmit to evolutionary distinct hosts; in these cases, the phylogenetic relationship of the hosts usually are close. READ MORE

4. 3. Separation of Nanoporous Silica Particles

   University essay from KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

   Author : Frida Preuss; Julia Asp; Sofia Larsson; Stephanie Kylington; [2020]
   Keywords : Monodispersity; particle size distribution; density gradient; centrifugation; silica;

   Abstract : In this study a sample of particles in a size region of 0.05-10 μm were run through a centrifugation process with the ambition to make it monodisperse. The product requirements were stated as follows, particles within the size range of 2 to 3.8 μm should be isolated and separated from the sample with a D90/D10 < 1. READ MORE

5. 4. PREPARATION AND CHARACTERIZATION OF BOVINE MEAT AND MILK FACTOR VESICLES/PARTICLES & IDENTIFICATION OF MSBI1 REP INTERACTING PROTEINS

   University essay from Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

   Author : Farhana Manzoor; [2020]
   Keywords : Bovine meat and milk factors; multiple sclerosis; immunoprecipitation co-immunoprecipitation; protein dimers; mass spectrometry; immunodetection; cesium chloride density gradient centrifugation; sodium dodecyl sulfate-polyacrylamide gel electrophoresis; vesicle; particle; interaction partner; pull-down assay.;

   Abstract : Introduction: Bovine meat and milk factors (BMMF) frequently isolated from bovine meat and milk, are discussed as putative causative agents for breast, colon and possibly other cancers. The hypothesis was formulated based on earlier correlative studies indicating that BMMFs could indirectly trigger inflammation, accumulate mutational events via reactive oxygen and nitrogen species production which eventually provokes tumor formation. READ MORE

6. 5. Development and evaluation of a pre-analytical device for liquid biopsy

   University essay from

   Author : Nicole Yacoub; [2018]
   Keywords : ;

   Abstract : In this project, a novel method to discover and monitor cancer was studied. Tumoureducated platelets (TEPs) have shown the ability to take up tumour-derived secretedmembrane vesicles, which contain tumour RNA. Therefore they are of great interestfor detecting cancer and could potentially work as biomarkers. READ MORE