Essays about: "HPAEC"

Found 5 essays containing the word HPAEC.

  1. 1. Geobacillus 7349 – A new potential biosurfactant producing microorganism

    University essay from Lunds universitet/Bioteknik (master); Lunds universitet/Bioteknik (CI)

    Author : Zhuoying Deng; [2023]
    Keywords : biosurfactant; rhamnolipids; HPLC; HPAEC-PAD; bacteria growth; downstream purification; liquid-liquid extraction; biotechnology; Chemistry;

    Abstract : The main aim of this master project is to find out whether strain Geobacillus 7349 can produce biosurfactant during their growth by extraction and characterization of the product. The other goal is to optimize the cultivation condition of the strain. READ MORE

  3. 2. Oligosaccharide Analysis - Assessment of the DNS Method and Specification of Glucans Processed with CGTase

    University essay from Lunds universitet/Bioteknik; Lunds universitet/Bioteknik (master)

    Author : Matilda Johansson; [2022]
    Keywords : CGTase; DNS; Chromatography; HPAEC; HPAE; Carbohydrate; Carbohydrates; Glucans; Cyclodextrin glycosyltransferase; Biotechnology; Bioteknik; Flory; Enza; Enzyme; Technology and Engineering;

    Abstract : In this study, the reagent 3,5-dinitrosalicylic acid (DNS) and high-performance anion exchange chromatography (HPAEC) were used for carbohydrate characterisation. It was revealed that the DNS reaction is not exclusive to reducing ends as commonly thought, but rather that the reagent reacts with other parts of the carbohydrate molecules as well. READ MORE

  4. 3. Comparison of Corn and Rye Arabinoxylans for the Production of Bio-based Materials

    University essay from KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

    Author : Chen Chen; [2020]
    Keywords : cereal bran; subcritical water extraction; arabinoxylans; enzymatic crosslinking; hydrogel; spannmålskli; extraktion av subkritiskt vatten; arabinoxylaner; enzymatisk tvärbindning; hydrogel;

    Abstract : Enzymes and subcritical water can be used for the extraction of hemicelluloses from cereal by-products, making the processes eco-friendly. The polysaccharides extracted from cereal by-products can be used as matrices for development of materials for various applications. READ MORE

  5. 4. Method development for identification of N-linked glycans by high performance anion exchange chromatography with pulsed amperometric detection and time of flight mass spectrometry

    University essay from Institutionen för fysik, kemi och biologi

    Author : Johanna Alm; [2011]
    Keywords : N-linked glycans; HPAEC-PAD; desalting glycans; TOF;

    Abstract : In the biopharmaceutical industry, identification of glycans in a glycoprotein is a regulatory requirement and is a part of the characterization of the protein. Glycans are constructed of several monosaccharides linked together. N-linked glycans, which have been studied in this project, are attached to the nitrogen atom in asparagine. READ MORE

  6. 5. Mutational engineering, growth selection, expression, purification and crystallization of ligand bound endo-beta-N-acetylglucosaminidase T (Endo-T) from Hypocrea jecorina

    University essay from SLU/Dept. of Molecular Biology (until 131231)

    Author : Andreas Digre; [2010]
    Keywords : Endo-T; Endo-beta-N-acetylglucosaminidase; Deglycosylation; EC 3.2.1.96; Crystallography; Ligand bound structure; High mannose N-glycan; HPAEC-PAD;

    Abstract : Recently, one of the first fungal-expressed, deglycosylating, Endo-beta-N-acetylglucosaminidases was found in the extracellular medium of soft-rot ascomycete Hypocrea jecorina (a.k.a. Trichoderma reesei) belonging to glycoside hydrolase family 18 (GH18). READ MORE