Essays about: "His-tag cleavage"

Found 3 essays containing the words His-tag cleavage.

1. 1. Optimizing the Production of Aurora Kinase A and Validation of Constructs with different Sequential Lengths

   University essay from Linköpings universitet/Kemi

   Author : Linnea Pierre; [2022]
   Keywords : Protein; Protein chemistry; Aurora Kinase A; Aurora A; Cancer; Structural Biology; Cancer research;

   Abstract : Aurora Kinase A is a kinase involved in multiple signaling pathways and interactions during mitosis, making it an essential kinase that deregulated causes cancer diseases in affected patients. Structural research shows mainly static snapshots of possible conformations of the partly disordered protein. READ MORE

3. 2. Production and characterisation of a chlamydial antigen candidate for vaccine trials

   University essay from Uppsala universitet/Molekylär biomimetik

   Author : Therese Koivula; [2021]
   Keywords : Chlamydia trachomatis; Immunology; Vaccine; Optimizing protein expression in E. coli; Optimizing protein purification; IMAC; His-tag cleavage; Buffer exchange; Chlamydial antigen;

   Abstract : The bacterium Chlamydia trachomatis is the leading cause of bacterial sexually transmitted infection worldwide. When left untreated, chlamydial infections can lead to severe complications, such as infertility. READ MORE

4. 3. Cloning and Expression of TBEV NS4A in A549 cells

   University essay from Örebro universitet/Institutionen för medicinska vetenskaper

   Author : Lovisa Gerdhem; [2019]
   Keywords : ;

   Abstract : Background: Tick borne Encephalitis Virus (TBEV), the cause of the fatal Central Nervous System (CNS) infection Tick Borne Encephalitis (TBE), belongs to the family of Flaviviridae and the genusFlavivirus. In order to replicate themselves, they cause the formation of a replication complex, which is nestled inside vesicles of the Endoplasmic Reticulum (ER) membrane to protect the RNA from host defense mechanisms. READ MORE