Essays about: "IPTG"

Found 4 essays containing the word IPTG.

1. 1. Trade-offs in CRISPR Immunity against Mobile Genetic Elements

   University essay from Uppsala universitet/Molekylär systembiologi

   Author : Johanna Cederblad; [2022]
   Keywords : CRISPR; microfluidics; fluorescent microscopy; RP4 conjugative plasmid;

   Abstract : The prokaryotic adaptive immune system CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) is a defense mechanism that helps to protect the prokaryotic cell from invading mobile genetic elements. This project was performed at Uppsala University and served to answer whether the expression of Cascade, which is part of the CRISPR defense system, will have a negative effect on the cell that expresses it and to also determine whether the CRISPR defense system is effective enough to stop the spread of a conjugative plasmid. READ MORE

3. 2. Optimising IPTG and Lactose Induction of Recombinant Expression with Flow-based Online Analysis

   University essay from Lunds universitet/Bioteknik (master)

   Author : Balder Werin; [2019]
   Keywords : IPTG; Lactose; Induction; Recombinant expression; Flow injection analysis; TnBgl3B; Online analysis; Cultivation; Enzymatic assay; Flow-based; Biotechnology; Bioteknik; Technology and Engineering;

   Abstract : The induction of recombinant expression in bioprocesses is associated with high costs, but still suffers from unoptimised standard procedures with high use of IPTG. A flow-based online method for enzymatic analysis of recombinant expression in E. READ MORE

4. 3. Odorant binding protein and olfactory receptors: plausible role as detectors in an odorant biosensor

   University essay from KTH/Skolan för kemivetenskap (CHE)

   Author : Linda Bengtsson; [2011]
   Keywords : odorant binding protein; olfaction; olfactory receptor; secretion; signal sequence;

   Abstract : The development of an inexpensive, portable, stable, sensitive and selective biosensor for detection of odorants is a daunting task. Here, we hypothesized the development of a detector layer composed of the protein groups; the olfactory receptors (ORs) and the odorant binding proteins (OBPs), known to bind odorants in animal sensing. READ MORE

5. 4. Cloning of the functional domains of TSP-1 for protein expression

   University essay from Institutionen för medicinsk biokemi och mikrobiologi

   Author : Shadi Zangi; [2009]
   Keywords : Thrombospondin-1; Angiogenesis; cloning; protein expression;

   Abstract : Thrombospondin-1 (TSP-1) is a multifunctional extracellular matrix glycoprotein that is released from platelets α-granule to regulate angiogenesis process. TSP-1 is well-known as an inhibitory factor of angiogenesis that binds to angiogenesis stimulating factors, for example fibroblast growth factor 2 (FGF-2), vascular endothelial growth factor (VEGF) and hepatocyte growth factor/scatter factor (HGF/SF), to inhibit angiogenesis. READ MORE