Essays about: "In-Cell ELISA"

Found 5 essays containing the words In-Cell ELISA.

1. 1. Design, production and in vitro evaluation of brain shuttle scFc-scFv8D3 affinity-mutants

   University essay from Uppsala universitet/Institutionen för farmaci

   Author : Jonathan Efverström; [2023]
   Keywords : Blood-brain barrier; In-Cell BBB-trans assay; mTfR1; Transferrin receptor; scFc-scFv8D3; Blod-hjärn barriären; mTfR1; Transferrin receptorn; scFc-scFv8D3;

   Abstract : The blood brain barrier (BBB) is a challenge when developing monoclonal antibodies for neurological diseases as antibodies do not cross the BBB making brain distribution challenging. An antibody construct which binds mTfR1 to pass the BBB, scFc-scFv8D3, have previously been developed. READ MORE

3. 2. Development of a combined In-Cell ELISA and flow cytometry method for quantification of uptake of PEGylated nanoparticles by Raw264.7 and HepG2 cells

   University essay from Lunds universitet/Tillämpad biokemi

   Author : Evelina Folkesson; [2016]
   Keywords : HepG2; Raw264.7; PEGylation; PEG; cancer; nanoparticles; In-Cell ELISA; flow cytometry; applied biochemistry; tillämpad biokemi; Technology and Engineering;

   Abstract : Cancer is one of today’s most common causes of human mortality. For long, chemotherapeutics has been a conventional treatment of the disease, but due to its low precision and high frequency of side effects, this treatment has been highly debated. READ MORE

4. 3. Effects of 1,25(OH)2D3 on Smad2 Activity in PC-3 Prostate Cancer Cells

   University essay from Institutionen för vård och natur

   Author : Anette Stahel; [2009]
   Keywords : prostate cancer; PC-3; vitamin D; 1; 25 OH 2D3; TGFβ; Smad2; Smad3;

   Abstract : The vitamin D metabolite 1,25(OH)2D3 has long been known to inhibit growth of prostate cancer cells and this mainly through a VDR-mediated pathway controlling target gene expression, resulting in cell cycle arrest, apoptosis and differentiation. Another major way inwhich 1,25(OH)2D3 inhibits cell growth in prostate cancer is via membrane-initiated steroid signalling, which triggers activation of signal cascades upon steroid binding to a receptor complex, leading to induction of genes regulating cell growth, proliferation and apoptosis. READ MORE

5. 4. 1,25(OH)2D3 Initially Reduces TGFβ Activity in PC-3 Prostate Cancer Cells

   University essay from Institutionen för vård och natur

   Author : Anette Stahel; [2008]
   Keywords : prostate cancer; PC-3; proliferation; growth inhibition; vitamin D; 1; 25 OH 2D3; TGFβ; Smad2; Smad3;

   Abstract : The vitamin D metabolite 1,25(OH)2D3 has long been known to inhibit growth of prostate cancer cells and this mainly through a VDR-mediated pathway controlling target gene expression, resulting in cell cycle arrest, apoptosis and differentiation. Another major way in which 1,25(OH)2D3 inhibits cell growth in prostate cancer is via membrane-initiated steroid signalling, which triggers activation of signal cascades upon steroid binding to a receptor complex, leading to induction of genes regulating cell growth, proliferation and apoptosis. READ MORE

6. 5. Development and characterization of Mantle Cell Lymphoma specific IgGs

   University essay from Institutionen för fysik, kemi och biologi

   Author : Katarina Gärdefors; [2008]
   Keywords : Mantle cell lymphoma MCL ; Sox11; human recombinant antibodies; single chain variable fragment scFv ; IgG.;

   Abstract : Mantle cell lymphoma (MCL) is one of several sub-types of B-cell lymphomas. The malignancy is very aggressive and average survival time is short. The hallmark of MCL is over expression of cyclin D1, however about 15% of all MCL cases do not display this over expression and are easily misdiagnosed. READ MORE