Essays about: "Jonbyteskromatografi"

Found 4 essays containing the word Jonbyteskromatografi.

  1. 1. Preparative chromatographyfor modified oligonucleotides : Method development for modified oligonucleotides, fromanalytical to preparative chromatography

    University essay from Karlstads universitet

    Author : Rebecka Jasinski; [2021]
    Keywords : oligonucleotides; preparative chromatography; scaling-up; ion-pair chromatography; dibutylamine; oligonukleotider; preparativ kromatografi; uppskalning; jonparskromatografi; dibutylamin;

    Abstract : Synthetic oligonucleotides, which are short strings of DNA or RNA, are a grooving area of importance for the pharmaceutical industry and for companies that manufacture diagnostic components. The manufacturing process of synthetic oligonucleotides involves many complex processes that use separation and purification techniques like ion-exchange chromatography, ion-pair reversed phase chromatography and ultra-performance liquid chromatography. READ MORE

  2. 2. The dependence of chromatographic conditions for separation of oligonucleotides in different AEX-HPLC columns

    University essay from KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

    Author : Julia Nylander; [2020]
    Keywords : ion exchange chromatography; oligonucleotides; anion exchange column; pH; HPLC; Jonbyteskromatografi; oligonukleotider; anjonbytarkolonn; pH; HPLC;

    Abstract : Oligonucleotides (ONs) are widely used in different applications in life science, forensic, in i.e. family tree DNA test for humans and in diagnostic applications in several fields. READ MORE

  3. 3. Cloning, expression and production of Vicia faba Leghemoglobin B

    University essay from Lunds universitet/Tillämpad biokemi

    Author : Gustav Lindéus; [2016]
    Keywords : blood substitute; fermentation; gene technology; Leghemoglobin; hemoglobin; Vicia faba; applied biochemistry; tillämpad biokemi; Biology and Life Sciences;

    Abstract : In this thesis a preliminary evaluation of the potential of a leghemoglobin (Lb) for the development of a blood substitute in humans, resembling hemoglobin (Hb) in red blood cells, was conducted. Leghemoglobin B (VfLbB), a gene from Vicia faba, was cloned into a pET-DEST42 vector, using the Gateway™ recombination technology. READ MORE

  4. 4. Selective Quantification of Co-Eluting Proteins by Means of Partial Least Squares

    University essay from Lunds universitet/Kemiteknik (CI)

    Author : Sofia Henrysson; [2014]
    Keywords : Technology and Engineering;

    Abstract : The aim of this thesis is to create a model of the correlation between absorbance and concentration of co-eluting proteins. A separation using the chromatograph system ÄKTA from GE-Health care of BSA,Lysozyme and IgG was performed. Absorbance was measured at three different wave lengths. READ MORE