Essays about: "Multiplex Polymerase Chain Reaction"

Found 5 essays containing the words Multiplex Polymerase Chain Reaction.

  1. 1. Optimization of a multiplex ARMS-PCR for detection of the primary mutations causing Leber’s hereditary optic neuropath

    University essay from Uppsala universitet/Institutionen för kvinnors och barns hälsa

    Author : Klara Jäder; [2020]
    Keywords : Allele-specific PCR; LHON; Multiplex Polymerase Chain Reaction; mitochondrial DNA; single nucleotide polymorphism;

    Abstract : Leber’s hereditary optic neuropathy (LHON) is a genetic disease that causes the patients to become blind, first in one eye and then the other, around the ages of 10-75 years. The disease is caused by mutations in the mitochondrial DNA, which disturbs the respiratory chain leading to the deterioration of the retinal ganglion cells. READ MORE

  2. 2. Optimization of a Multiplex PCR-RFLP Method Used for Detection of Three Primary Mutations in Leber’s Hereditary Optic Neuropathy Patients

    University essay from Uppsala universitet/Institutionen för kvinnors och barns hälsa

    Author : Emilia Nord; [2020]
    Keywords : LHON; Restriction enzyme; Multiplex PCR; Mutation detection; Mitochondrial mutation;

    Abstract : Leber’s hereditary optic neuropathy (LHON) is the most commonly inherited disease that causes blindness in one or both eyes, with a minimum prevalence of 1 in 31 000 in the northeast of England. What causes LHON is not fully known but three mitochondrial mutations, G3460A, G11778A, and T14484C, have been identified in over 95 % of all LHON patients. READ MORE

  3. 3. Detection of poleroviruses causing virus yellows in sugar beet

    University essay from SLU/Dept. Of Plant Biology

    Author : Josefin Pettersson; [2020]
    Keywords : beet chlorosis virus; beet mild yellowing virus; sugar beet; Sweden; turnip yellows virus;

    Abstract : Sugar beet is a crop cultivated for production of sugar. Many diseases can cause yield loss of sugar beet, where some are caused by viruses. Virus yellows is caused by several viruses and is visible as yellowing of sugar beet leaves. These viruses are transmitted by aphids and can be found across the world in temperate regions. READ MORE

  4. 4. Compartmentmentalized immuno-sequencing (cI-Seq) : identification of immune complex interactions

    University essay from KTH/Genteknologi

    Author : Susanne Johansson; [2016]
    Keywords : PCR; Immuno-PCR; Sequencing; emPCR; Compartmetalization; Antibodies Immun-complex; Luminex;

    Abstract : Today, a lot of proteomic research is aimed at discovering disease specific proteins. This requires theavailability of high-throughput, ultra-sensitive protein detection methods. Compartmentalized immunosequencing(cI-Seq) is a proximity-independent immuno-polymerase chain reaction (IPCR) based proteindetection method. READ MORE

  5. 5. Optimization and validation of a triplex real-time PCR assay for thermotolerant Campylobacter species associated with foodborne disease

    University essay from SLU/Dept. of Microbiology

    Author : Maria Karlsson; [2013]
    Keywords : thermotolerant Campylobacter; multiplex real-time PCR; 16S rRNA; hipO; cadF;

    Abstract : The genus Campylobacter is globally recognised as the leading bacterial cause of human foodborne gastroenteritis. Every year around 8000 Swedes are infected by Campylobacter. Most people are infected by thermotolerant Campylobacter species, commonly C. jejuni and C. READ MORE