Essays about: "Next Generation Sequencing"

Showing result 1 - 5 of 66 essays containing the words Next Generation Sequencing.

  1. 1. CRISPR Screen to Identify Genes Regulating Melanoma Cell Invasiveness

    University essay from KTH/Proteinvetenskap

    Author : Emma Kroon; [2023]
    Keywords : CRISPR-Cas9; gene knockout; viral packaging; transduction; invasionstest; CRISPR-Cas9; genknockout; viruspackning; transduction; invasion assay;

    Abstract : Maligna cancerceller har förmågan att sprida sig okontrollerat i kroppen och metastasera till avlägsna organ. Kritiskt i metastaserings processen är cancercellers förmåga att invadera närliggande vävnader innan de sprider sig vidare ut i kroppen. READ MORE

  3. 2. Multiple-Emitter Super-resolution Imaging using the Alternating Descent Conditional Gradient Method

    University essay from Lunds universitet/Beräkningsbiologi och biologisk fysik - Genomgår omorganisation; Lunds universitet/Institutionen för astronomi och teoretisk fysik - Genomgår omorganisation

    Author : Dolev Illouz; [2023]
    Keywords : super-resolution; alternating descent conditional gradient method; ADCG; multiple-emitter localization; nanoscale imaging; single molecule tracking; Physics and Astronomy;

    Abstract : This thesis examines the state-of-the-art 2D super-resolution technique alternating descent conditional gradient (ADCG) method's ability to accurately localize fluorophores in diffraction-limited single molecule images (SMI) and analyze the impact of pre-processing and post-processing modules on ADCG's fluorophore localization. A synthetic dataset obtained from the 2013 Grand Challenge localization microscopy and a temporally linked dataset obtained from an unpublished set of Optical DNA mapping experiments performed by Jonathan Jeffet at the NanoBioPhotonix Lab at Tel-Aviv University were initially segmented to extract their noise parameters. READ MORE

  4. 3. Establishing novel approaches for assessing safety of genome-editing-based therapeutics

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Noorul Shaheen Sheikh Farid; [2023]
    Keywords : ;

    Abstract : Genome editing based medicines are a new therapeutic modality being developed. They show great promise for a spectrum of genetic diseases lacking conventional treatment strategies. A major obstacle that prevents such therapies to reach the clinic is their safety. READ MORE

  5. 4. PAMPaR: Parallel Analysis of miRNAs, Proteins and RNAs. Dynamic Profiles of miRNAs, mRNAs and Specific Proteins in Mouse Embryonic Stem Cells Differentiating into Neural Precursors

    University essay from KTH/Proteinvetenskap

    Author : Amelie Carlström; [2023]
    Keywords : PAMPaR; SPARC; Proximity extension assay; Neuronal differentiation; Mouse embryonic stem cells; PAMPaR; SPARC; Proximity extension assay; Neuronal differentiering; embryonala musstamceller;

    Abstract : MikroRNA-molekyler (miRNA) är viktiga icke-kodande RNA-molekyler för post-transkriptionell reglering av proteinkodande gener. De har även effekter på många cellulära processer såsom differentiering. De biosyntetiska reaktioner som skapar miRNA involverar proteiner som själva regleras av miRNA. READ MORE

  6. 5. Development of a simplified and cost effective norovirus capsid typing method using next generation sequencing

    University essay from

    Author : Ronnie Eriksson; [2023]
    Keywords : PCR; Foodborne virus; Food safety; Illumina; NGS;

    Abstract : Human noroviruses are a major cause of acute gastroenteritis worldwide and can betransmitted through consumption of contaminated raw food. Shellfish like oysters can becontaminated by human sewage during production and accumulate multiple Norovirus strainsin low concentrations. READ MORE