Essays about: "Nicolas Rochereau"

Found 1 essay containing the words Nicolas Rochereau.

  1. 1. Rye cell wall β-glucosidase: subcloning, expression and purification of recombinant protein from E.coli

    University essay from Institutionen för livsvetenskaper

    Author : Nicolas Rochereau; [2007]
    Keywords : rye; defense system; β-glucosidase; subcloning; his-tag; thioredoxin-tag; E.coli; Ni-NTA; western blot;

    Abstract : Several plant defense systems consist of enzymes that act on glucosides and produce a toxic compound. In the intact plant tissue the substrate and enzyme are kept apart. The system studied here consists of the substrate 2-O-β-D-glucopyranosyl-4-dihydroxy-1,4-benzoxazin-3-one and the enzyme glucan 1,3-β-glucosidase in rye. READ MORE