Essays about: "Pull-down assay"

Found 4 essays containing the words Pull-down assay.

  1. 1. Mass Spectrometric Virus Detection with Multiplex Assay

    University essay from KTH/Proteinvetenskap

    Author : Sebastian Augustinsson; [2022]
    Keywords : Multiplex assay; Binder; Antigen; Cloning; Pull-down assay; Targeted detection; Mass spectrometry;

    Abstract : Syftet med projektet var att utveckla en multiplexanalys för att detektera antigen från SARS-CoV-2, influensa och respiratoriskt syncytial virus genom att använda en masspektrometrisk metod som involverar antigenspecifika bindare. Bindarna klonades, renades och biotinylerades innan de användes i en analys utvecklad genom en målinriktad metod som involverade antigenerna. READ MORE

  3. 2. PREPARATION AND CHARACTERIZATION OF BOVINE MEAT AND MILK FACTOR VESICLES/PARTICLES & IDENTIFICATION OF MSBI1 REP INTERACTING PROTEINS

    University essay from Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

    Author : Farhana Manzoor; [2020]
    Keywords : Bovine meat and milk factors; multiple sclerosis; immunoprecipitation co-immunoprecipitation; protein dimers; mass spectrometry; immunodetection; cesium chloride density gradient centrifugation; sodium dodecyl sulfate-polyacrylamide gel electrophoresis; vesicle; particle; interaction partner; pull-down assay.;

    Abstract : Introduction: Bovine meat and milk factors (BMMF) frequently isolated from bovine meat and milk, are discussed as putative causative agents for breast, colon and possibly other cancers. The hypothesis was formulated based on earlier correlative studies indicating that BMMFs could indirectly trigger inflammation, accumulate mutational events via reactive oxygen and nitrogen species production which eventually provokes tumor formation. READ MORE

  4. 3. Identification of protein-interaction partners of ZBED6

    University essay from SLU/Dept. of Animal Breeding and Genetics

    Author : Mi Zhou; [2012]
    Keywords : ZBED6; transcription factor; BAC TransgeneOmics; tandem affinity purification; protein interaction;

    Abstract : ZBED6 was recently discovered as a novel transcriptional repressor of IGF2 because a mutation disrupting one of its binding sites in porcine IGF2 intron 3 leads to greater postnatal IGF2 expression in skeletal muscle and makes pig grow more muscle. ZBED6 is unique to placental mammals and derived from domesticated DNA transposon. READ MORE

  5. 4. Integrin Signalling

    University essay from Institutionen för medicinsk biokemi och mikrobiologi

    Author : Roselien Schelfaut; [2005]
    Keywords : Cell surface receptors - integrins - Ras - PI3K;

    Abstract : Integrins are receptors presented on most cells. By binding ligand they can generate signalling pathways inside the cell. Those pathways are a linkage to proteins in the cytosol. It is known that tumor cells can survive and proliferate in the absence of a solid support while normal cells need to be bound to ligand. READ MORE