Essays about: "Quantification of bacteria"

Showing result 11 - 15 of 23 essays containing the words Quantification of bacteria.

  1. 11. CUSTOMIZED QUANTIFICATION OF HOST CELL PROTEIN WITH BIO-LAYER INTERFEROMETRY

    University essay from Lunds universitet/Teknisk mikrobiologi

    Author : Eva Sjöblom; [2020]
    Keywords : BLI; HCP; CHO; yeast; host cell protein; bio-layer interferometry; immunoassays; quantification; chinese hamster ovary; ELISA; applied microbiology; teknisk mikrobiologi; Biology and Life Sciences; Technology and Engineering;

    Abstract : Host cell protein (HCP) is quantified in the purification steps of biopharmaceutical production and is part of ensuring the purity of the final drug product. The gold standard method for HCP quantification is enzyme-linked immunosorbent assay (ELISA), but a new approach is proposed here with fully customized bio-layer interferometry assays for yeast and chinese hamster ovary (CHO) cells. READ MORE

  3. 12. Investigating the methane producing pathway in lab-scale biogas reactors subjected to sequential increase of ammonium and daily acetate-pulsing

    University essay from Linköpings universitet/Institutionen för fysik, kemi och biologi

    Author : Sofia Moberg; [2020]
    Keywords : Syntrophic acetate oxidation; syntrophic acetate oxidizing bacteria; Wood-Ljungdahl pathway;

    Abstract : Syntrophic acetate oxidizing bacteria convert acetate into hydrogen and carbon dioxide and through the mutualistic syntrophic partnership with methanogens the products are further converted to methane in biogas processes operating at high ammonia concentrations. There is very little known about SAOBs, only five have been characterized and had their genome analyzed. READ MORE

  4. 13. Spatial and temporal changes in microbial community composition in a full-scale woodchip bioreactor for treating mine water

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Felicia Wallnäs; [2020]
    Keywords : ;

    Abstract : Incomplete detonation of nitrogen-based explosives can lead to abundant levels of nitrate in mine groundwater. The possibility of reducing nitrogen levels from the wastewater through denitrification, anammox and DNRA has been investigated using a full-scale bioreactor. The bioreactor is situated subsurface and is filled with pine woodchips. READ MORE

  5. 14. Evaluation of next-generation sequencing as a tool for determining the presence of pathogens in clinical samples

    University essay from Högskolan i Skövde/Institutionen för biovetenskap

    Author : Alexander Kokkonen; [2019]
    Keywords : NGS; next generation sequencing; clinical microbiology; systems biology;

    Abstract : Metagenomic sequencing is an increasingly popular way of determining microbial diversity from environmental and clinical samples. By specifically targeting the 16S rRNA gene found in all bacteria, classifications of pathogens can be determined based on the variable and conserved regions found in the gene. READ MORE

  6. 15. Polyvinylalcohol-carbazate (PVAC) inhibits bacteria growth

    University essay from Uppsala universitet/Institutionen för kvinnors och barns hälsa

    Author : Jakob Syk; [2019]
    Keywords : Turbidity; spectrophotometric assay; FITC; antibodies; qPCR;

    Abstract : Abstract Introduction This study evaluated the effect of the polymer polyvinylalcohol-carbazate (PVAC) on the bacteria Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis and Pseudomonas aeruginosa. PVAC is a polymer with a carbazate moiety that neutralizes free aldehydes and has shown great promise in stabilizing erythrocytes during long term storage. READ MORE