Essays about: "RPLC"

Found 2 essays containing the word RPLC.

1. 1. Investigation of Adsorption and Retention of Charged Compounds In RPLC

   University essay from Karlstads universitet/Institutionen för ingenjörs- och kemivetenskaper (from 2013)

   Author : Emma Fryxelius; [2022]
   Keywords : adsorption; adsorption isotherm; charged compounds; promethazine hydrochloride; propranolol hydrochloride; mixed-mode; retention; RPLC;

   Abstract : The adsorption isotherm of two weak bases, Promethazine hydrochloride and Propranolol hydrochloride, were determined with isocratic reversed-phase liquid chromatography, with a 60 w% methanol in 20 mM sodium acetate buffer pH 4 as the mobile phase, and calculated by the elution by characteristic points method. The data obtained from the method were then fitted into the Langmuir isotherm and the electrostatically modified Langmuir. READ MORE

3. 2. The impact of various chromatographic conditions on the separation of modified and unmodified oligonucleotides

   University essay from Karlstads universitet

   Author : Lewis Frazer; [2021]
   Keywords : Oligonucleotide; Modified oligonucleotides; Ion-pair chromatography; Chromatography; Analytical chemistry; TEAA; DBA; Chemistry; Oligonukleotid; Modifierade oligonukleotider; Jonpars kromatografi; Kromatografi; Analytisk kemi; TEAA; DBA; Kemi;

   Abstract : In this study, the effects of certain chromatographic conditions on various modified and unmodified oligonucleotides were investigated. At the forefront of this study was the investigation of a new Ion-pair Reversed-phase liquid chromatography (IP-RPLC) method, that had the potential to replace a previously established triethylammonium acetate (TEAA) IP-RPLC method developed for oligonucleotide separations. READ MORE