Essays about: "RT qPCR"

Showing result 1 - 5 of 54 essays containing the words RT qPCR.

  1. 1. The Quantitative Expression of Transport Proteins SLC38A1-11 in Nerve Cell Development

    University essay from Uppsala universitet/Institutionen för farmaceutisk biovetenskap

    Author : Maryam Dinar; [2023]
    Keywords : SLC38A1; SNAT1; SLC38A2; SNAT2; SLC38A3; SNAT3; SLC38A4; SNAT4; SLC38A5; SNAT5; SLC38A6; SNAT6; SLC38A7; SNAT7; SLC38A8; SNAT8; SLC38A9; SNAT9; SLC38A10; SNAT10; SLC38A11; SNAT11; SLC; SLC38; SNATs; SNAT; RT-qPCR; GABA; glutamine; glutamate;

    Abstract : Solute carriers consist of over 400 genes and 66 subfamilies and are the largest group of membrane bound transporters. They facilitate movement of many molecules through cells and organelles and are crucial for upholding many key functions in the human body. They are also involved in the pathophysiology of several diseases. READ MORE

  3. 2. Developing Wastewater-based Early Warning System for the Detection of Disease Outbreaks and Emerging Variants with focus on SARS-CoV-2

    University essay from KTH/Industriell bioteknologi

    Author : Ayda Kiyar; [2023]
    Keywords : wastewater-based epidemiology; early warning system; RT-qPCR; normalization; SARS-CoV-2; avloppsvattenbaserad epidemiologi; förvarningssystem; RT-qPCR; normalisering;

    Abstract : Under covid-19-pandemin har avloppsvattenbaserad epidemiologi (WBE) använts i stor utsträckning som ett komplement till kliniska tester över många delar av världen. Detta projekt syftade till att detektera och kvantifiera belastningen av Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) i avloppsvattenprover med hjälp av Revers transkriptas kvantitativ polymeraskedjereaktion (RT-qPCR). READ MORE

  4. 3. Evaluation of manual and robotic techniques for Micro-RNA extraction in early sepsis diagnosis

    University essay from Högskolan i Skövde/Institutionen för biovetenskap

    Author : Tjorven Plontke; [2023]
    Keywords : ;

    Abstract : Sepsis is a life-threatening organ dysfunction caused by the spread of infectious diseases throughout the body, resulting in a dysfunctional immune system response. It includes a wide range of symptomsa ffecting multiple organs, making accurate diagnosis challenging. READ MORE

  5. 4. Genetic manipulation to improve efficacy of dendric cell adoptive immunotherapy against cancer in dogs

    University essay from Karlstads universitet

    Author : Felicia Berglund; [2023]
    Keywords : Adoptive immunotherapi;

    Abstract : To improve the efficacy of the dendritic cell vaccine Alv B DC from Alv B, the PD-L1 expression in cancer cells was attempted to be reduced through a transfection with a custom designed siRNA. Before transfecting the dendritic cells, the siRNA functionality had to be tested through flow cytometry, that resulted in negative results and therefore led to a RT-qPCR protocol that indicated that the siRNA was functional. READ MORE

  6. 5. Investigating the crosstalk between estrogen receptor beta in colorectal cancer and tumor-associated macrophages

    University essay from KTH/Proteinvetenskap

    Author : Alicia Bodin; [2023]
    Keywords : estrogen; macrophages; colorectal cancer; qPCR; cell culture; östrogen; makrofager; tjocktarmscancer; qPCR; cellodling;

    Abstract : Tjock-och ändtarmscancer (kolorektalcancer) är den tredje vanligaste cancertypen och den näst vanligaste cancer-relaterade dödsorsaken i världen. Östrogen har visat sig ha en skyddande roll mot kolorektalcancer och östrogenreceptor beta är den dominerande östrogenreceptorn i normalt kolonepitel. READ MORE