Essays about: "Shotgun proteomics"

Showing result 1 - 5 of 9 essays containing the words Shotgun proteomics.

1. 1. Combining output from MS-based proteomics search engines using spectrum predictors

   University essay from KTH/Proteinvetenskap

   Author : William Hadd; [2022]
   Keywords : Mass spectrometry; Proteomics; machine learning; search engines; Masspektrometri; Proteomik; Maskininlärning; Transformers; Sökmotorer;

   Abstract : Masspektrometri (MS) är en analysmetod som indikerar provers kemiska sammansättning. Provernas innehåll fragmenteras och joniseras, varefter jonernas förhållande mellan massa och laddning (m/z) och sammanlagda intensiteter mäts i form av ett masspektrum. READ MORE

3. 2. Identification of metabolite-protein interactions among enzymes of the Calvin Cycle in a CO2-fixing bacterium

   University essay from KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

   Author : Emil Sporre; [2020]
   Keywords : Cupriavidus necator; chemolithoautotroph; limited proteolysis; Calvin Cycle; carbon fixation; proteomics; allosteric regulation; metabolite-protein interactions.;

   Abstract : The Calvin – Benson cycle is the most widespread metabolic pathway capable of fixing CO2 in nature and a target of very high interest to metabolic engineers worldwide. In this study, 12 metabolites (ATP, AMP, NADP, NADPH, 2PG, 3PGA, FBP, RuBP, PEP, AKG, Ac-CoA and phenylalanine) were tested for protein – metabolite interactions against the proteome of Cupriavidus necator (previously Ralstonia eutropha) in the hopes of finding potential examples of allosteric regulation of the Calvin – Benson cycle. READ MORE

4. 3. Proteomics Study of a Designed Nanoparticle-Protein Corona Made of Animal Model Plasma

   University essay from Linköpings universitet/Institutionen för fysik, kemi och biologi

   Author : Elin Nilsson; [2020]
   Keywords : Nanoparticle; Protein corona; Nanoparticle-protein corona; SUrface proteomics; Safety; Targeting; and Uptake;

   Abstract : Nanoparticles are currently finding increasing use as drug delivery systems in the treatment of cancer and other disorders. When nanoparticles are introduced into body fluids, they adsorb proteins forming a coating called protein corona. READ MORE

5. 4. Prediction of Ranking of Chromatographic Retention Times using a Convolutional Network

   University essay from KTH/Skolan för elektroteknik och datavetenskap (EECS)

   Author : Daniel Kruczek; [2018]
   Keywords : Shotgun proteomics; retention time; retention time prediction; neural networks; convolutional neural networks;

   Abstract : In shotgun proteomics, the liquid chromatography step is used to separate peptides in order to analyze as few as possible at the same time in the mass spectrometry step. Each peptide has a retention time, that is how long it takes to pass through the chromatography column. READ MORE

6. 5. Unlocking the Proteome of Archival Tissue Collections : Proteomic Analysis of Urinary Bladder Cancer Tissues using Mass Spectrometry-based Proteomics

   University essay from Uppsala universitet/Analytisk kemi

   Author : Aleš Holfeld; [2017]
   Keywords : ;

   Abstract : Formalin-fixed and paraffin-embedded (FFPE) and optimal cutting temperature (OCT)-embedded frozen tissue samples have recently gained interest as they exist in vast repositories worldwide and represent a valuable resource for clinical studies. However, unlocking the proteome of these archival tissues has encountered many challenges. READ MORE