Essays about: "Site-directed"

Showing result 1 - 5 of 17 essays containing the word Site-directed.

1. 1. OPTIMIZATION OF CLONE SELECTION PLATFORM FOR PRODUCTION OF RECOMBINANT PROTEINS

   University essay from Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

   Author : Basel Yousef; [2024]
   Keywords : ;

   Abstract : Background  Biologics are type of proteins that can be produced by expression in a foreign host for medical healthcare applications in humans. Monoclonal antibodies (mAbs) are type of biologics that are designed to specifically bind to one target, with a purpose to inhibit a certain molecule that could be associated with a disease process. READ MORE

3. 2. Killer action of Spok homologue in Fusarium vanattenii : Investigation through site directed mutagenesis

   University essay from Uppsala universitet/Institutionen för organismbiologi; Uppsala universitet/Institutionen för biologisk grundutbildning

   Author : Samuel Jorayev; [2023]
   Keywords : Spok genes; FuSpok; Meiotic Driver; Fusarium; transposons;

   Abstract : The Spok genes are a group of selfish genetic elements in the fungus Podespora anserina which kills spores lacking them. These Spoks function through a toxin and resistance domain. Homologues of these have been found across other species such as in the Fusarium genus. READ MORE

4. 3. Structure-based design of novel pharmacological tools for the A3 adenosine G protein-coupled receptor

   University essay from Uppsala universitet/Institutionen för läkemedelskemi

   Author : Ashish Tamhankar; [2021]
   Keywords : ;

   Abstract : Adenosine receptors (ARs) are a family belonging to the GPCR superfamily, involved with multiple physiological processes and widely distributed throughout the body. In this study, we focus on the A3 AR due to its vast scale applications in various pathophysiological conditions such as inflammation, pain, allergic asthma, and cancer chemotherapy. READ MORE

5. 4. Increased expression of therapeutic proteins by identification of 3'-UTRs from high expressing genes in CHO cells

   University essay from Linköpings universitet/Institutionen för fysik, kemi och biologi

   Author : Alexander Westlund; [2019]
   Keywords : Mammalian cell; CHO; E. coli; protein expression; plasmid; transformation; Fc-fusion protein; flow cytometry; FACS; transfection; mRNA; UTR; gene engineering;

   Abstract : Therapeutic proteins, a.k.a. biopharmaceuticals, are most commonly produced in expression systems derived from Chinese Hamstery Ovary (CHO) cells, thanks to great capacity of post-translational modifications like secretation, folding and glycosylation. READ MORE

6. 5. Characterization of DNA binding of the two zinc finger domains of transcription factor zBED6

   University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

   Author : Alexander Taubert; [2019]
   Keywords : transcription factor; zinc finger; zBED6;

   Abstract : The zinc finger protein, zBED6, is a transcriptional regulator of IGF2 along with hundreds of other genes relating to development and growth. Studies on the growth of commercially bred pigs discovered a single nucleotide substitution in the third intron of IGF2 which disrupts the binding of zBED6 and is responsible for the three-fold upregulation of IGF2 in skeletal muscle. READ MORE