Essays about: "Southern blot"

Found 4 essays containing the words Southern blot.

  1. 1. Design, expression and purification of virus-like particles derived from metagenomic studies : Virus-like Particles (VLP) of novel Partitiviridae species, Hubei.PLV 11, and novel Soutern pygmy squid flavilike virus were designed, expressed using the bac-to-bac expression system and then pruified using various methods

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Diyar Ayranci; [2021]
    Keywords : Virus; virus-like particles; VLP. Western Blot; SDS-PAGE; Chromatography; partitivrus; partitiviridae; flaviviridae; flavivirus; Hubei partitilike virus; Southern Pygmy Squid flavilike virus; protein purification; protein characterization; PCR; Cloning; metagenomic; strucutral biology; cryo-em; bac-to-bac expression system; vaccines; arbovirus; arthropod-borne virus;

    Abstract : Viruses are entities which are made of a few genes and are reliant on obligate parasitism to propagate. Due to the obligate connection to their hosts, virus evolution is constrained to the type of host. Viruses however do transmit to evolutionary distinct hosts; in these cases, the phylogenetic relationship of the hosts usually are close. READ MORE

  3. 2. Evaluation of transgenic lines of field pepperweed (Lepidium campestre L.)

    University essay from SLU/Dept. of Plant Breeding (from 130101)

    Author : Rikard Holst; [2013]
    Keywords : field pepperweed; transgenic; WRI1; oil content; PCR; Southern blot; vernalization;

    Abstract : The worldwide industrial demand of energy, often in form of fossil fuel, is continuously growing and at the same time these resources are coming to an end (Hamamre, 2013). The interest and focus in renewable sources are then more important than ever and plants are suggested to be a good alternative for production of raw material for industrial purposes (Nilsson et al. READ MORE

  4. 3. The effects of muscle damaging electrically stimulated contractions and ibuprofen on muscle regeneration and telomere lengths in healthy sedentary males

    University essay from Örebro universitet/Institutionen för hälsovetenskap och medicin

    Author : Mathias Ekstrand; [2011]
    Keywords : telomeres; NSAIDs; muscle regeneration; muscle damage; electrical stimulation;

    Abstract : Introduction: The effect of electrical stimulation on muscle degeneration and regeneration is largely unknown and it has not been studied in conjunction with telomeres. The consumption of non-steroidal anti-inflammatory drugs (NSAIDs) is widespread in athletes and the general population when faced with muscle soreness or injury. READ MORE

  5. 4. Transformation of E. coli and Agrobacterium and molecular analysis of transformed apple (Malus domestica) rootstock A2

    University essay from SLU/Dept. Of Plant Breeding

    Author : Carin Emanuelsson; [2007]
    Keywords : plant biotechnology; transformation; A2; rol C; GA2ox;

    Abstract : This project includes two parts. The first part was transformation of Escherichia coli and Agrobacterium tumefaciens with a vector containing the GA2ox gene. The second part was molecular analyses of the apple (Malus domestica) rootstock A2 transformed with the rolC gene. The rolC and GA2ox genes are two potential genes for dwarfing plants. READ MORE