Essays about: "T7 RNA polymerase"

Found 2 essays containing the words T7 RNA polymerase.

1. 1. Technoeconomic analysis of T7 RNA polymerase manufacturing in E.coli for South Africa

   University essay from Lunds universitet/Kemiteknik (CI)

   Author : Alicia Lundh; [2022]
   Keywords : T7 RNA polymerase; enzyme manufacturing; mRNA vaccine manufacturing; chemical engineering; Technology and Engineering;

   Abstract : The enzyme, T7 RNA polymerase (T7 RNAP), is one of the most expensive raw materials required for mRNA vaccine production. As part of establishing South African manufacturing of mRNA vaccine, it is of interest to investigate the feasibility and profitability of South African manufacturing of T7 RNAP. READ MORE

3. 2. Rolling circle transcription on smallest size double stranded DNA minicircles

   University essay from Institutionen för biologisk grundutbildning

   Author : Anders Kristoffersson; [2010]
   Keywords : RNA polymerase; RNAP T7; DNA nanotechnology; molecular motor; real time monitoring of transcription; 2’ O methyl RNA; molecular beacon; minicircle;

   Abstract : The RNA polymerase T7 is utilized as a component of motor complexes in DNA nanotechnology due to its high promotor specificity, the lack of external transcription factors and its very high processivity, but there is no experience of its application on small double stranded DNA circles. Circular templates from 210 to 126 bp in circumference sharing a common promotor termination motif were synthesized and transcription was monitored at end point on gel and in real time with a 2’ O methyl RNA molecular beacon. READ MORE