Essays about: "Western blot analysis"

Showing result 1 - 5 of 27 essays containing the words Western blot analysis.

  1. 1. Endothelial Protein C Receptor : Expression in the murine kidney

    University essay from Uppsala universitet/Institutionen för farmaceutisk biovetenskap

    Author : Lina Molin; [2022]
    Keywords : Endothelial protein C receptor; murine kidney; murine kidney endothelial cells; glomerular cells; flow cytometry; western blot; dot blot; SDS-PAGE; murine kidney tissue;

    Abstract : This thesis aims to investigate if the endothelial protein C receptor is expressed in the murine kidney. This was done by performing flow cytometry and Western blot analysis on cultivated murine kidney endothelial cells (mKECs) as well as SDS-PAGE and Western blot analysis on murine kidney tissue. READ MORE

  3. 2. VISUALIZING CATESTATIN AND CHROMOGRANIN A : An Antibody Validation

    University essay from Uppsala universitet/Institutionen för medicinsk cellbiologi

    Author : Johan William-Olsson; [2022]
    Keywords : Diabetes; immunology; western blot; flow cytometry; immunofluorescence;

    Abstract : Chromogranin A (CgA) and its cleavage product Catestatin (CST) are two very interesting proteins, especially due to their possible involvement in the development of type 1 diabetes. There are currently only a few primary antibodies against CgA and CST, and the ones that do exist are often only recommended for use on one method. READ MORE

  4. 3. DINCH and DINP Plasticizers Alter Lipid Metabolism in 3T3-L1 cells

    University essay from Örebro universitet/Institutionen för naturvetenskap och teknik

    Author : Isabel Häggblom; [2022]
    Keywords : ;

    Abstract : Di(isononyl)cyclohexane – 1,2 dicarboxylate (DINCH) and Diisononyl phthalate (DINP) are high molecular weight compounds used as plasticizers. DINCH and DINP use on the market have been increasing recently to replace previously used phthalate plasticizers in polymeric products. READ MORE

  5. 4. The role of exosomes in sarcoidosis

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Elli Mouchtaridi; [2021]
    Keywords : ;

    Abstract : Sarcoidosis is a systemic inflammatory disease mostly affecting the lungs, marked by the presence of granulomas and the accumulation of interferon gamma (IFNγ)-producing T cells in the affected organs. Extracellular vesicles (EVs) are membranous vesicles with a size range of 50 nm to 5000 nm, with exosomes ranging from 50 to 150 nm. READ MORE

  6. 5. Functional characterization of the SLC38 transporters SNAT6, SNAT8 and SNAT10 using CRISPR-Cas9 knockout in vitro

    University essay from Uppsala universitet/Institutionen för farmaceutisk biovetenskap

    Author : Alfred Holmberg; [2020]
    Keywords : SNAT6; SNAT8; SNAT10; SLC38; SLC38a6; SLC38a8; SLC38a10; CRISPR-Cas9; crispr; glutamate-glutamine cycle; ggc; glutamate; glutamine; gaba; hek293;

    Abstract : There are currently over 430 known SLC transporters, over 30% of which have an unknown function. Compared to other transporter gene families, the SLC genes are relatively understudied with many orphan genes. READ MORE