Essays about: "anion-exchange chromatography"

Showing result 1 - 5 of 17 essays containing the words anion-exchange chromatography.

  1. 1. Navigating Large-Scale Plasmid DNA Purification : A Recommendation of Current and Future Downstream Purification Solutions

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Matilda Eriksson; Alva Wells; Maria Frey; Lisa Johansson; Gabriel Pettersson; David Sjöberg; [2023]
    Keywords : Plasmid purification; pDNA; large-scale chromatography;

    Abstract : Previous small-scale methods for plasmid DNA (pDNA) purification fail to meet theindustry’s demand for sufficient quantities. Greater volumes of bacterial lysates are a consequence of larger volumetric fermentations and traditional large-scale down-stream purification processes have some disadvantages and limitations. READ MORE

  3. 2. Remediation of per- and polyfluoroalkyl substances (PFAS) in contaminated water by sorption to pine- and spruce bark

    University essay from Örebro universitet/Institutionen för naturvetenskap och teknik

    Author : Rebecka Ayranci Dahlberg; [2023]
    Keywords : Per- and polyfluoroalkyl substances; sorption; pine bark; spruce bark; ultra-short-chain PFAS; short-and long-chain PFAS; elemental analysis; field-test;

    Abstract : PFAS are anthropogenic substances used in many different industrial operations and products because of their unique properties. Due to their negative impact on human health and environmental bioaccumulative characteristics different authorities have addressed the issue. In Sweden the limit is4 ng/L for four different PFAS in drinking water. READ MORE

  4. 3. Comparison of purifying DARPins using conventional methods and self-cleaving affinity chromatography

    University essay from Uppsala universitet/Biokemi

    Author : Sofia Larsson; [2023]
    Keywords : ;

    Abstract : An important issue in pharmaceutical industry is to develop processes to purify proteins. Thereare many purification methods that can be used for this purpose, but all methods have differentconstraints. READ MORE

  5. 4. Characterization of ulvan polysaccharide degrading enzymes from Wenyingzhuangia fucanilytica

    University essay from Lunds universitet/Bioteknik; Lunds universitet/Bioteknik (master)

    Author : Erik Apelqvist; [2022]
    Keywords : Enzyme; Enzymatic characterization; Recombinant protein producaiton; Protien purification; E. coli; W. fucanilytica; Transformation; Overexpression; Seaweed; Ulvan; Polysaccharides; biotechnology; Biology and Life Sciences;

    Abstract : The aim of this thesis was to fully characterize the enzyme WFp-105, including bioinformatic analysis, structural modeling, full activity and stability characterization and product determination. As well as for the enzyme WFp-37; optimize protein production in regard to expression strains and cultivation temperature, analyzing the genomic context as well as performing an initial activity detection. READ MORE

  6. 5. Phytic acid in faba bean : the effect of pH, soaking time and heat-treatment to reduce the phytic acid content offaba bean

    University essay from SLU/Department of Molecular Sciences

    Author : Rebecka Sterner; [2022]
    Keywords : Plant-based food; Vicia faba; phytic acid; soaking treatment; anion-exchange chromatography; colorimetric method;

    Abstract : To feed a globally growing population, a challenging shift in the usage of today’s food system is required. This includes a shift towards more sustainable human diets, with increased levels of protein from plant-based sources. Faba bean (Vicia faba L. READ MORE