Essays about: "cloning culture"

Showing result 1 - 5 of 8 essays containing the words cloning culture.

1. 1. Recombinant production of spider silk for enzymatic phosphorylation

   University essay from KTH/Proteinvetenskap

   Author : Kristel Jaiu; [2022]
   Keywords : Caddisfly larval silk; FN-4RepCT; phosphorylation; protein kinase A; recombinant spider silk; Nattsländesilke; FN-4RepCT; fosforylering; proteinkinas A; rekombinant spindeltrådsilke;

   Abstract : Ett rekombinant framställt miniatyrspindelsilkeprotein, funktionaliserat med ett Arg-Gly-Asp-motiv som finns i fibronektin, FN-4RepCT, har använts för många olika bioteknologiska tillämpningar, som vävnadsteknik och cellodling, i form av fibrer, filmer och skum. FN-4RepCT kan produceras i Escherichia coli, och det interagerar spontant med sig självt och bildar fibrer under fysiologiska förhållanden. READ MORE

3. 2. Design, expression and purification of virus-like particles derived from metagenomic studies : Virus-like Particles (VLP) of novel Partitiviridae species, Hubei.PLV 11, and novel Soutern pygmy squid flavilike virus were designed, expressed using the bac-to-bac expression system and then pruified using various methods

   University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

   Author : Diyar Ayranci; [2021]
   Keywords : Virus; virus-like particles; VLP. Western Blot; SDS-PAGE; Chromatography; partitivrus; partitiviridae; flaviviridae; flavivirus; Hubei partitilike virus; Southern Pygmy Squid flavilike virus; protein purification; protein characterization; PCR; Cloning; metagenomic; strucutral biology; cryo-em; bac-to-bac expression system; vaccines; arbovirus; arthropod-borne virus;

   Abstract : Viruses are entities which are made of a few genes and are reliant on obligate parasitism to propagate. Due to the obligate connection to their hosts, virus evolution is constrained to the type of host. Viruses however do transmit to evolutionary distinct hosts; in these cases, the phylogenetic relationship of the hosts usually are close. READ MORE

4. 3. Protecting positions of power and privileges : The Racial Glass Ceiling in the UK Civil Society Sector

   University essay from Lunds universitet/Socialhögskolan

   Author : Stela Kosova; [2020]
   Keywords : social closure; cloning culture; everyday racism; obstacles; BAME; career trajectory; Social Sciences;

   Abstract : As institutional racism and the underrepresentation of BAME have become world-wide central issues, the question of equal opportunities for upward mobility in the labour market and social mobility for this particular community are at the forefront, including the UK context. Evidence suggests that BAME have unequal access to opportunities for career progression in the workplace and that upward mobility is inextricably linked to obstacles at every stage of their career in comparison to their white counterparts. READ MORE

5. 4. Purification, functional characterization and crystallization of the PerR peroxide sensor from Saccharopolyspora erythraea

   University essay from Uppsala universitet/Strukturbiologi

   Author : Grim Elison Kalman; [2019]
   Keywords : structural biology; cell culture; protein expression; genetic engineering; spectroscopy; X-ray crystallography; small angle X-ray scattering; SAXS; transcription factor; PerR; metal binding; antibiotics; Saccharopolyspora erythraea; S. erythraea; soil bacterium; actinobacteria; actinomycetes; oxidative stress; peroxide stress; structure determination; characterization; stabilization; peroxide sensor; iron sensor; manganese sensor; metalloprotein; metal ion homeostasis; metal ion; prokaryotic; strukturbiologi; cellodling; proteinuttryck; genteknik; spektroskopi; röntgenkristallografi; småvinkel-röntgenspridning; SAXS; transkriptionsfaktor; PerR; metallbindande; antibiotika; Saccharopolyspora erythraea; S. erythraea; jordartsbakterie; Aktinobakterier; Actinobacteria; Actinomycetes; oxidativ stress; peroxid stress; strukturbestämning; karaktärisering; stabilisering; peroxidsensor; järnsensor; mangansensor; metalljonshomeostas; metalljoner; prokaryot;

   Abstract : This report summarizes the work on the cloning, expression, and purification of PerR, a metal sensing regulator from Saccharopolyspora erythraea and the subsequent characterization using small angle X-ray scattering and other biochemical methods. The report aims to provide an insight into prokaryotic metal homeostasis, provide a better understanding of how PerR works and provide valuable information for the continued work on the crystallization of PerR. READ MORE

6. 5. Molecular characterization of the faecal fungal flora in healthy horses

   University essay from SLU/Dept. of Animal Nutrition and Management

   Author : Ehsan Khajouei; [2013]
   Keywords : Anaerobic fungi; Equine; Eukaryotic microorganism; Fungal ITS gene; Gut microbiota; Horse; 18S rRNA gene; Neocallimastigaceae family;

   Abstract : There is a lack of knowledge on the composition and dynamics of the intestinal micro-eukaryotes of horse and to date their functional role for the health and physiology of the host has been described merely to some extent. Particularly, no culture-independent surveys of the dynamics of the intestinal fungal flora in horse are available. READ MORE