Essays about: "gene cloning"

Showing result 1 - 5 of 33 essays containing the words gene cloning.

  1. 1. Potential Application of Multiplex Automated Genome Engineering (MAGE) and One-Step Curing Plasmid System for Environmental Cambodian Enterobacterial Isolates

    University essay from Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

    Author : Olivia Alexandra; [2021]
    Keywords : Antimicrobial resistance; plasmid curing; environmental isolates; recombineering; enterobacteria;

    Abstract : Antimicrobial resistance (AMR) is concerning because it limits antimicrobial drug treatment options. AMR occurs by the overuse and misuse of antimicrobial drugs. In environmental settings, AMR can disseminate from places of high use, which leads to increased exposure to humans and animals. READ MORE

  3. 2. Automated pipeline for processing fluorescence calcium imaging microscopy data and electrophysiological measurements from 3D brain organoids

    University essay from Lunds universitet/Examensarbeten i bioinformatik

    Author : Mattis Knulst; [2021]
    Keywords : Biology and Life Sciences;

    Abstract : Motivation: 3D brain organoids are biological models of human brain development from which a rich body of data can be extracted that allows for insights about neuronal dynamics. Several tools, such as fluorescence calcium imaging microscopy and electrophysiological recordings are being used to assess how 3D brain organoid dynamics recapitulate what is known from the human brain. READ MORE

  4. 3. Methods for production of recombinant enzymes for collagen degradation

    University essay from Lunds universitet/Tillämpad biokemi; Lunds universitet/Beräkningskemi

    Author : Tea Eriksson; Elin Su; [2020]
    Keywords : Collagenase; Subtilisin; Collagen; Enzymatic hydrolysis; Enzymes; Collagen degradation; Enzyme production; Cloning; Gene optimization; Protein expression; Technology and Engineering;

    Abstract : In this thesis eight enzymes have been identified with documented ability to degrade collagen or its hydrolyzed products. Collagen can be found in skin, bones, and connective tissue. Hydrolyzed collagen peptides are utilized in food, disease treatment, skincare and training supplements. READ MORE

  5. 4. Construction of a Fusion Gene : to anchor a truncated version of the inflammatory receptor NLRP3 to the cell membrane

    University essay from Högskolan i Skövde/Institutionen för biovetenskap

    Author : Miguel Ángel Postigo Peláez; [2019]
    Keywords : NLRP3; Inflammation; Fusion gene; PCR; Fusion protein;

    Abstract : Inflammasomes are a group of protein complex that regulate inflammation throughcomplex signal transduction, although their specific mechanisms and structures have notbeen fully described. As the protein that kickstarts assembly of a type of inflammasome,NLRP3 is a key regulator of inflammation and may play a relevant role in the developmentof inflammatory diseases. READ MORE

  6. 5. Increased expression of proteins in CHO cells by identification of signal peptides for improved secretion of translated proteins

    University essay from Linköpings universitet/Teknisk biologi

    Author : Malin Strannermyr; [2018]
    Keywords : Chinese hamster ovary; signal peptide; protein expression; plasmid; library; gene; Fc-fusion; monoclonal antibody; E. coli; flow cytometry; transfection; transformation;

    Abstract : Main purpose of this study was to increase protein expression in Chinese hamster ovary (CHO) cells by improving protein secretion of translated proteins. The goal was to find signal peptides from the screening of signal peptide libraries for improvement of protein secretion using a CHO-cell express selection system. READ MORE