Essays about: "gradient elution"

Showing result 1 - 5 of 8 essays containing the words gradient elution.

1. 1. Investigation of Adsorption and Retention of Charged Compounds In RPLC

   University essay from Karlstads universitet/Institutionen för ingenjörs- och kemivetenskaper (from 2013)

   Author : Emma Fryxelius; [2022]
   Keywords : adsorption; adsorption isotherm; charged compounds; promethazine hydrochloride; propranolol hydrochloride; mixed-mode; retention; RPLC;

   Abstract : The adsorption isotherm of two weak bases, Promethazine hydrochloride and Propranolol hydrochloride, were determined with isocratic reversed-phase liquid chromatography, with a 60 w% methanol in 20 mM sodium acetate buffer pH 4 as the mobile phase, and calculated by the elution by characteristic points method. The data obtained from the method were then fitted into the Langmuir isotherm and the electrostatically modified Langmuir. READ MORE

3. 2. Analytical Methods for High Molecular Weight UV Stabilizers

   University essay from KTH/Kemi

   Author : Elin Ljungström; [2022]
   Keywords : hindered amine light stabilizers; light stabilizers; polyolefins; LC-MS; HPLC; hindrade aminljusstabilisatorer; ljusstabilisatorer; polyolefiner; LC-MS; HPLC;

   Abstract : Utomhusprodukter gjorda i plast är sårbara för nedbrytning på grund av UV-energi från solljus. För att stoppa denna fotonedbrytning används UV-stabilisatorer i plastmaterial; en av huvudkategorierna är hindrade aminljusstabilisatorer (HALS, hindered amine light stabilizers). READ MORE

4. 3. The dependence of chromatographic conditions for separation of oligonucleotides in different AEX-HPLC columns

   University essay from KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

   Author : Julia Nylander; [2020]
   Keywords : ion exchange chromatography; oligonucleotides; anion exchange column; pH; HPLC; Jonbyteskromatografi; oligonukleotider; anjonbytarkolonn; pH; HPLC;

   Abstract : Oligonucleotides (ONs) are widely used in different applications in life science, forensic, in i.e. family tree DNA test for humans and in diagnostic applications in several fields. READ MORE

5. 4. Iterative Learning Control for Preparative Chromatography

   University essay from Lunds universitet/Kemiteknik (CI)

   Author : Daniel Espinoza; [2020]
   Keywords : Chemical Engineering; Kemiteknik; Chromatography; Automatic Process Control; Iterative Learning Control; ILC; Simulation; Protein Purification; Python; Ion-Exchange; Automation; Batch Processing; Downstream Processing; Technology and Engineering;

   Abstract : There is a desire for a fully automated downstream process in pharmaceutical protein production. One part of the downstreaming process is preparative chromatography. A good separation between the product and other proteins, as well as good productivity, are desired. READ MORE

6. 5. Identification of Trypsin Digested Transferrin using HPLC and MALDI-MS

   University essay from KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

   Author : Weyni Ghebreamlak; [2019]
   Keywords : Transferrin; Glycopeptides; Digestion; HPLC; MALDI-MS;

   Abstract : In this project, separation of trypsin digested transferrin (Tf) has been studied, using a RP HPLC- UV system equipped with a C18 column. 0.1% TFA/MQ-water and 90% MeOH were used as mobile phase A and mobile phase B, respectively. READ MORE