Essays about: "high-performance size exclusion chromatography"

Showing result 1 - 5 of 6 essays containing the words high-performance size exclusion chromatography.

1. 1. Optimization of two high-performance size-exclusion chromatography methods with diode-array detection for protein quantification and purity assessment

   University essay from Lunds universitet/Centrum för analys och syntes

   Author : Camilla Lane; [2023]
   Keywords : Method qualification; Protein concentration; Protein Purity; High Performance Size exclusion chromatography; Quality control; Analytical chemistry; Biology and Life Sciences;

   Abstract : Background: There is a need to develop methods of determining concentration and purity for two new affinity ligands, protein T and protein H, in order to ensure the quality of an industrial production of said proteins. Aims: To develop a High Performance Size Exclusion Chromatography, HPSEC, method to determine concentration and purity of protein T and to improve an existing HPSEC method for concentration and purity determination of protein H that show selectivity, display linearity between peak area and injection amount and give repeatable results. READ MORE

3. 2. Swedish wheat in the changing climate : screening for stable spring wheat genotypes from 2017 and 2018 with focus on protein quality for bread-making

   University essay from SLU/Dept. of Plant Breeding (from 130101)

   Author : Axel Belsing; [2021]
   Keywords : wheat; gluten protein; bread-making quality; stability; climate variation; breeding; SE-HPLC; micro-structure;

   Abstract : In recent years, wheat production and protein quality in wheat have been affected by climatic changes in Sweden and in many other parts of the world. Protein quality of wheat, determining the bread-making quality of flour, is a parameter sensitive to climate fluctuations and its stability has not been studied so far. READ MORE

4. 3. Advanced control of chromatography systems for automated analysis of monoclonal antibodies

   University essay from Lunds universitet/Kemiteknik (CI)

   Author : Jerry Guan; [2021]
   Keywords : Chemical engineering; Kemiteknik; Chromatography; Analysis; HPLC; Python; Automatic process control; Monoclonal Antibodies; Antibody purification; Technology and Engineering;

   Abstract : Nedströmsprocesser för rening av biologiska läkemedel som monoklona antikroppar rör sig från batch-processer till kontinuerliga, och vidare till helt automatiska, kontinuerliga nedströmsprocsser. En del av visionen för automatiska reningsprocesser är möjligheten att automatiskt analysera från olika steg av reningen. READ MORE

5. 4. Purification, activity assays and crystallization of GtCel45A - a small cellulase enzyme from the brown-rot fungus Gloeophyllum trabeum expressed in Aspergillus nidulans

   University essay from Uppsala universitet/Strukturbiologi

   Author : Louise Fitkin; [2021]
   Keywords : ;

   Abstract : Cellulose is the most abundant polymer on earth. It is one of the main components in lignocellulosic biomass, which has great potential as a renewable energy source. To utilize the biomass, for instance in biofuel production, cellulose needs to be degraded. READ MORE

6. 5. Implementation of the mille-feuille nanofilter paper in the virus removal filtration of IgY purified from chicken egg yolk

   University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

   Author : Michelle Dahlman; [2020]
   Keywords : ;

   Abstract : The purpose of this thesis was to test the mille-feuille nanofilter paper for a virus removal filtration from a potential protein-based nutraceutical. Therefore, chicken IgY was purified from egg yolk, with PEG precipitation for crude fractionation followed by thiophilic adsorption chromatography. READ MORE