Essays about: "his-tag"

Showing result 1 - 5 of 16 essays containing the word his-tag.

1. 1. Expression and purification of pyruvate transporting human mitochondrial pyruvate carrier (MPC) protein complex for structural studies

   University essay from Lunds universitet/Examensarbeten i molekylärbiologi

   Author : Markus Wendler; [2022]
   Keywords : Biology and Life Sciences;

   Abstract : Pyruvate is one of the main molecules needed for the citric acid cycle, which produces energy in the form of ATP. The citric acid cycle reaction occurs in the mitochondrial matrix, and for pyruvate to participate in the reaction, it needs to be transported from the cytosol across the mitochondrial membranes into the mitochondrial matrix. READ MORE

3. 2. Optimizing the Production of Aurora Kinase A and Validation of Constructs with different Sequential Lengths

   University essay from Linköpings universitet/Kemi

   Author : Linnea Pierre; [2022]
   Keywords : Protein; Protein chemistry; Aurora Kinase A; Aurora A; Cancer; Structural Biology; Cancer research;

   Abstract : Aurora Kinase A is a kinase involved in multiple signaling pathways and interactions during mitosis, making it an essential kinase that deregulated causes cancer diseases in affected patients. Structural research shows mainly static snapshots of possible conformations of the partly disordered protein. READ MORE

4. 3. Production and characterisation of a chlamydial antigen candidate for vaccine trials

   University essay from Uppsala universitet/Molekylär biomimetik

   Author : Therese Koivula; [2021]
   Keywords : Chlamydia trachomatis; Immunology; Vaccine; Optimizing protein expression in E. coli; Optimizing protein purification; IMAC; His-tag cleavage; Buffer exchange; Chlamydial antigen;

   Abstract : The bacterium Chlamydia trachomatis is the leading cause of bacterial sexually transmitted infection worldwide. When left untreated, chlamydial infections can lead to severe complications, such as infertility. READ MORE

5. 4. Design, expression and purification of virus-like particles derived from metagenomic studies : Virus-like Particles (VLP) of novel Partitiviridae species, Hubei.PLV 11, and novel Soutern pygmy squid flavilike virus were designed, expressed using the bac-to-bac expression system and then pruified using various methods

   University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

   Author : Diyar Ayranci; [2021]
   Keywords : Virus; virus-like particles; VLP. Western Blot; SDS-PAGE; Chromatography; partitivrus; partitiviridae; flaviviridae; flavivirus; Hubei partitilike virus; Southern Pygmy Squid flavilike virus; protein purification; protein characterization; PCR; Cloning; metagenomic; strucutral biology; cryo-em; bac-to-bac expression system; vaccines; arbovirus; arthropod-borne virus;

   Abstract : Viruses are entities which are made of a few genes and are reliant on obligate parasitism to propagate. Due to the obligate connection to their hosts, virus evolution is constrained to the type of host. Viruses however do transmit to evolutionary distinct hosts; in these cases, the phylogenetic relationship of the hosts usually are close. READ MORE

6. 5. Biosensor based on immobilized amine transaminase for detection of amphetamine

   University essay from KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

   Author : Clara Öh; [2020]
   Keywords : amine transaminases ATA ; enzyme immobilization; biosensor; covalent immobilization; ionic immobilization; amphetamine; histidine-tagged enzyme; Poly methyl methacrylate PMMA ;

   Abstract : Amine transaminases (ATA) catalyse the transfer of an amino group from one molecule and replaces a ketone or aldehyde with the amino group, the amino group on the amino-donor is replaced with a ketone or aldehyde. This enzyme, ATA from Chromobacterium violaceum, has previously been used to catalyse the reaction involving amphetamine, therefore, it might be possible to use this enzyme to convert amphetamine and the product absorbs in the UV spectrum and can therefore be measured spectrophotometrically. READ MORE