Essays about: "in situ sequencing"

Showing result 1 - 5 of 9 essays containing the words in situ sequencing.

  1. 1. Ovary spheroid formation and quality assessment using two different protocols

    University essay from KTH/Proteinvetenskap

    Author : Nikki Wallius Hiltunen; [2023]
    Keywords : ovary; fertility; spheroid; Biosilk™; three-layer gradient system; äggstock; fertilitet; sfäroid; Biosilk™; trelagers gradientsystem;

    Abstract : Redan innan födseln fastställs kvinnors fertilitet, som karakteriseras av ett begränsat antal äggblåsor, så kallade folliklar. Beståndet av folliklar minskar naturligt med åldern, samtidigt som kvinnors fertilitet beror på deras steroid- och äggproduktion. READ MORE

  3. 2. Developing Automated Cell Segmentation Models Intended for MERFISH Analysis of the Cardiac Tissue by Deploying Supervised Machine Learning Algorithms

    University essay from KTH/Kemi

    Author : Julia Rune; [2023]
    Keywords : Cell Segmentation; Cellpose; Supervised Machine Learning; MERFISH; Heart Failure; Cellsegmentering; Cellpose; Övervakad Maskininlärning; MERFISH; Hjärtsvikt;

    Abstract : Följande studie behandlar utvecklandet av automatiserade cellsegmenteringsmodeller med avsikt att identifiera gränser mellan celler i hjärtvävnad. Syftet är att möjliggöra analys av data genererad från multiplexed error-robust in situ hybridization (MERFISH). READ MORE

  4. 3. Developing saddleback and emperor tamarin SNP set for in situ genotyping

    University essay from Uppsala universitet/Institutionen för ekologi och genetik; Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Samantha López Clinton; [2022]
    Keywords : SNP genotyping; GT-Seq; conservation genomics; saddleback tamarin; emperor tamarin; Genotipado por SNPs; GT-seq; genómica para la conservación; pichico común; pichico emperador;

    Abstract : Many countries in the global south - which harbour the majority of the world’s biodiversity - face serious resource limitations and a lack of access to affordable sequencing services. Furthermore, biodiversity research and monitoring of non-model, threatened and/or cryptic species often relies on low-quality non-invasive genetic samples. READ MORE

  5. 4. Visualizing neuronal cell sub-populations using novel transgenic zebrafish lines.

    University essay from Uppsala universitet/Institutionen för medicinsk biokemi och mikrobiologi

    Author : Aikaterini Zafeiriou; [2021]
    Keywords : Zebrafish; CRISPR Cas9; locomotion; confocal microscopy; immunohistochemistry;

    Abstract : Zebrafish is a frequently used model organism with an array of transgenic lines that have been used indevelopmental and physiological studies. We aim to generate novel transgenic zebrafish reporter lines to study subpopulations of spinal neurons in vivo. READ MORE

  6. 5. Graph neural networks for spatial gene expression analysis of the developing human heart

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning; Uppsala universitet/Avdelningen för visuell information och interaktion

    Author : Xiao Yuan; [2020]
    Keywords : single-cell RNA sequencing; in situ sequencing; graph neural networks; deep learning; unsupervised learning;

    Abstract : Single-cell RNA sequencing and in situ sequencing were combined in a recent study of the developing human heart to explore the transcriptional landscape at three developmental stages. However, the method used in the study to create the spatial cellular maps has some limitations. READ MORE