Essays about: "in vivo kinetics"

Showing result 1 - 5 of 7 essays containing the words in vivo kinetics.

  1. 1. Single-particle tracking for direct measurements of Trigger Factor ribosome binding in live cells

    University essay from Uppsala universitet/Molekylärbiologi

    Author : Tora Hävermark; [2021]
    Keywords : Trigger Factor; Single-particle tracking; in vivo kinetics; co-translational polypeptide processing;

    Abstract : Trigger Factor (TF) is a prokaryotic chaperone protein that exerts its major chaperone activity while associated with translating ribosomes, assisting de novo folding of the emerging nascent chain. Although much is known about the kinetics behind TF-ribosome binding, most results are based on in vitro experiments which fail to mimic the cellular environment. READ MORE

  3. 2. Biochemical characterization of resurrected ancestral ammonia lyases

    University essay from KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

    Author : Albin Holmberg Larsson; [2019]
    Keywords : Ancestral sequence resurrection; Inborn errors of metabolism; Aromatic ammonia lyases; Enzyme kinetics; Thermostability;

    Abstract : This study set out to express, purify and characterize twelve ammonia lyase enzymes for potential application as a supplement to a treatment of an inborn error of metabolism disease. The DNA sequence for two wild-type ammonia lyases, three modified ammonia lyases and seven resurrected ancestral ammonia lyases had been synthesized and cloned in vectors. READ MORE

  4. 3. Fluorescent fusion proteins as probes to characterize tau fibril polymorphism

    University essay from Linköpings universitet/Kemi

    Author : Max Lindberg; [2019]
    Keywords : Alzheimer’s disease AD ; amyloids; FRET; tau; MAPT; fibrillation kinetics; seeding; fusion protein; EGFP; ECFP; EYFP; ThT; X-34; BTD14;

    Abstract : Alzheimer's disease (AD) is a large and growing problem and while we today lack a full understanding of this disease, we know that the protein tau and the amyloid fibrils it forms play a central role in its development. We also know that these fibrils can have different morphologies in different diseases and that fibrils produced in vitro not necessarily adopt any of the morphologies found in patients. READ MORE

  5. 4. Oralt administrerad hyaluronan : påverkan på hästars ledhälsa

    University essay from SLU/Dept. of Biomedical Sciences and Veterinary Public Health

    Author : Tina Hansen; [2018]
    Keywords : hyaluronan; häst; ledsjukdom; fodertillskott;

    Abstract : Hälta är ett vanligt förekommande problem hos våra svenska sport- och ridhästar och två tredjedelar av alla hältor uppges vara orsakade av ledsjukdom. Behandling av traumatisk artrit syftar till att dämpa inflammation och minska smärta samt att hämma nedbrytning och stimulera nybildning av ledbrosk. READ MORE

  6. 5. Development of a tissue-like protoporphyrin IX fluorescence phantom and performance of characterisation experiments

    University essay from Biomedicinsk instrumentteknik; Tekniska högskolan

    Author : Benjamin Kistler; [2013]
    Keywords : ;

    Abstract : Background and Objective: Fluorescence spectroscopy is a potential tool for brain tumour resection surgery. The main advantages of fluorescence spectroscopy are the objective fluorescence detection, possibility to quantify the signals and high sensitivity for instance to distinguish between healthy and neoplastic tissue. READ MORE