Essays about: "ion exchange chromatography"

Showing result 11 - 15 of 25 essays containing the words ion exchange chromatography.

  1. 11. Iterative Learning Control for Preparative Chromatography

    University essay from Lunds universitet/Kemiteknik (CI)

    Author : Daniel Espinoza; [2020]
    Keywords : Chemical Engineering; Kemiteknik; Chromatography; Automatic Process Control; Iterative Learning Control; ILC; Simulation; Protein Purification; Python; Ion-Exchange; Automation; Batch Processing; Downstream Processing; Technology and Engineering;

    Abstract : There is a desire for a fully automated downstream process in pharmaceutical protein production. One part of the downstreaming process is preparative chromatography. A good separation between the product and other proteins, as well as good productivity, are desired. READ MORE

  3. 12. Development of separation method for analysis of oligonucleotides using LC-UV/MS

    University essay from Uppsala universitet/Analytisk farmaceutisk kemi

    Author : Björs Ida; [2018]
    Keywords : Oligonucleotides; UPLC; liquid chromatography; time of flight; mass spectrometry; reversed-phase weak anion exchange; AcclaimTM Mixed Mode WAX-1; DNAPacTM RP; ACQUITY UPLC BEH C18;

    Abstract : Introduction Oligonucleotides are short nucleic acid chains, usually 19-27mer long. They bind to their corresponding chain, making a specific inhibition possible. In pharmaceuticals, this can be used to inhibit the expression of a gene or protein of interest. READ MORE

  4. 13. The Expression, Purification and Characterization of Ebola Virion Protein 24 and Karyopherin Alpha 5

    University essay from Uppsala universitet/Institutionen för kvinnors och barns hälsa

    Author : Marina Obaid; [2018]
    Keywords : Ebolavirus; filovirus; VP24; KPNA5; IMAC;

    Abstract : Ebolavirus (EBOV) is a single stranded RNA virus that causes haemorrhagic fever in humans and other mammals. The EBOV encodes 7 proteins, NP, L, VP30, VP35, VP40, GP and VP24. VP24 is believed to be one of the EBOV proteins that causes the extreme virulence of the pathogen. READ MORE

  5. 14. Comparison of two HPLC columns: An attempt to improve analysis of carbohydrate-deficient transferrin

    University essay from Uppsala universitet/Institutionen för kvinnors och barns hälsa

    Author : Ida Engström; [2018]
    Keywords : Reprospher; liquid chromatography; ion exchange chromatography; CDT; gradient elution;

    Abstract : 1ABSTRACTCarbohydrate-deficient transferrin (CDT) is a biomarker for excessive and long-running intake of alcohol. It is a form of transferrin called disialotransferrin that under normal circumstances is <2 % of total transferrin in human blood. An increase is seen when alcohol consumption exceeds450 g per week. READ MORE

  6. 15. Cloning, expression and production of Vicia faba Leghemoglobin B

    University essay from Lunds universitet/Tillämpad biokemi

    Author : Gustav Lindéus; [2016]
    Keywords : blood substitute; fermentation; gene technology; Leghemoglobin; hemoglobin; Vicia faba; applied biochemistry; tillämpad biokemi; Biology and Life Sciences;

    Abstract : In this thesis a preliminary evaluation of the potential of a leghemoglobin (Lb) for the development of a blood substitute in humans, resembling hemoglobin (Hb) in red blood cells, was conducted. Leghemoglobin B (VfLbB), a gene from Vicia faba, was cloned into a pET-DEST42 vector, using the Gateway™ recombination technology. READ MORE