Essays about: "ion-pair chromatography"

Showing result 1 - 5 of 6 essays containing the words ion-pair chromatography.

1. 1. Ion pair chromatography of carboxylic and sulfonic acids with trialkylamines : An investigation of the relationship between the adsorption isotherm of the IPR and the retention factor of the analyte

   University essay from Karlstads universitet/Institutionen för ingenjörs- och kemivetenskaper (from 2013)

   Author : Karl Kullberg; [2023]
   Keywords : ;

   Abstract : Ion pair chromatography has found many uses within analytical chemistry and understanding of the chromatographic process with an ion-pairing reagent have been studied since the 1970s. The adsorption of the Ion-pairing reagent to the stationary phase is understood to be important and many studies have been dedicated to looking at the adsorption isotherms of the earlier quaternary alkylammonium ions. READ MORE

3. 2. Exploratory study of ion pair liquid chromatography separation methods and sample preparation procedures – for quantification of amount 5’capped and uncapped mRNA

   University essay from Karlstads universitet/Institutionen för ingenjörs- och kemivetenskaper (from 2013)

   Author : Felicia Lindström; [2023]
   Keywords : ;

   Abstract : Using ion pair chromatography, separation and analysis of shorter mRNA fragments was possible. The aim of the study was to develop a separation method for quantitation of 5’capped versus uncapped mRNA. Cleavage of mRNA was performed by complementary probes and the enzyme RNase H which created shorter mRNA fragments containing the 5’ end. READ MORE

4. 3. A study of the adsorption isotherms of Ion-pair reagents

   University essay from Karlstads universitet

   Author : Mehmet Bilici; [2022]
   Keywords : Tributylamine; adsorption isotherm; liquid-liquid extraction; gas chromatography;

   Abstract : In this project, the optimization of obtaining adsorption isotherms for the ion pair reagent, tributylamine was tested. The goal was to have a better understanding of the chromatographic process when separating biomolecules, such as oligonucleotides. READ MORE

5. 4. A Comparative Analysis of Per- andPolyfluoroalkyl Substances (PFAS) and ExtractableOrganofluorine (EOF) Using Solid PhaseExtraction-Weak Anion Exchange and Ion PairExtraction in SerumMarichal SalamehSpring 2021Independent project

   University essay from Örebro universitet/Institutionen för naturvetenskap och teknik

   Author : Marichal Salameh; [2021]
   Keywords : Extractable organofluorine EOF ; Per- and polyfluoroalkyl substances PFAS ; horse serum; human serum; ion pair extraction IPE ; solid phase extraction - weak anion exchange SPE-WAX ; liquid chromatography tandem mass spectrometry LC-MS MS ; combustion ion chromatography CIC ;

   Abstract : Per- and polyfluorinated substances (PFAS) are compounds that consist of a carbon chainbackbone that is partially or entirely fluorinated, with an addition of a functional group. SomePFAS are known as persistent organic pollutants (POPs) and have therefore been drawing a lot ofattention as well as increased concerns. READ MORE

6. 5. Preparative chromatographyfor modified oligonucleotides : Method development for modified oligonucleotides, fromanalytical to preparative chromatography

   University essay from Karlstads universitet

   Author : Rebecka Jasinski; [2021]
   Keywords : oligonucleotides; preparative chromatography; scaling-up; ion-pair chromatography; dibutylamine; oligonukleotider; preparativ kromatografi; uppskalning; jonparskromatografi; dibutylamin;

   Abstract : Synthetic oligonucleotides, which are short strings of DNA or RNA, are a grooving area of importance for the pharmaceutical industry and for companies that manufacture diagnostic components. The manufacturing process of synthetic oligonucleotides involves many complex processes that use separation and purification techniques like ion-exchange chromatography, ion-pair reversed phase chromatography and ultra-performance liquid chromatography. READ MORE