Essays about: "isocratic elution"

Found 3 essays containing the words isocratic elution.

  1. 1. Investigation of Adsorption and Retention of Charged Compounds In RPLC

    University essay from Karlstads universitet/Institutionen för ingenjörs- och kemivetenskaper (from 2013)

    Author : Emma Fryxelius; [2022]
    Keywords : adsorption; adsorption isotherm; charged compounds; promethazine hydrochloride; propranolol hydrochloride; mixed-mode; retention; RPLC;

    Abstract : The adsorption isotherm of two weak bases, Promethazine hydrochloride and Propranolol hydrochloride, were determined with isocratic reversed-phase liquid chromatography, with a 60 w% methanol in 20 mM sodium acetate buffer pH 4 as the mobile phase, and calculated by the elution by characteristic points method. The data obtained from the method were then fitted into the Langmuir isotherm and the electrostatically modified Langmuir. READ MORE

  2. 2. Unspecific interactions in different batch variations of agarose based base matrices at different NaCl concentrations.

    University essay from Karlstads universitet/Institutionen för ingenjörs- och kemivetenskaper (from 2013)

    Author : Kejvan Ghofranpanah; [2022]
    Keywords : Lysozyme; hydrophobicity; size exclusion chromatography; NaCl concentrations; isocratic elution; Lysozym; hydrofobicitet; size exclusion chromatography; NaCl-koncentrationer; isokratisk eluering;

    Abstract : Size exclusion chromatography (SEC) separates molecules based on their actual size rather than their molecular weight. This indicates that the smaller a molecule is, the longer it will be held in the column since it can penetrate the pores more effectively than a bigger molecule, resulting in a longer retention time, whereas the larger molecule has a shorter retention time. READ MORE

  3. 3. Development of a Liquid Chromatography Method to Separate and Fractionate EPA’s 16 Priority Polycyclic Aromatic Hydrocarbons (PAHs)

    University essay from Örebro universitet/Institutionen för naturvetenskap och teknik

    Author : Tim Sinioja; [2016]
    Keywords : ;

    Abstract : Polycyclic Aromatic Hydrocarbons (PAHs) are persistent, toxic and carcinogenic environmental pollutants consisting of two or more aromatic rings. The estimation of the toxicity of PAHs can be challenging and time-consuming due to the complexity of PAH-mixtures found in the environment, for example at contaminated sites. READ MORE