Essays about: "liquid chromatography - time-of-flight mass spectrometry"

Showing result 1 - 5 of 7 essays containing the words liquid chromatography - time-of-flight mass spectrometry.

1. 1. Separation and identification of IgG glycopeptides using Capillary Electrophoresis and Ultra High-Performance Liquid Chromatography-Mass Spectrometry

   University essay from KTH/Kemi

   Author : Madeleine Lövås; [2022]
   Keywords : Immunoglobulin G; peptides; CE; UHPLC-MS; MALDI-TOF; Immunglobulin G; peptider; CE; UHPLC-MS; MALDI-TOF;

   Abstract : I den här studien har metoder för att separera immunoglobulin (IgG) utvecklats med hjälp av kapillärelektrofores (CE) och Ultra högupplösande vätskekromatografi-Mass spektrometri (UHPLC-MS). Vid analysen av peptider från IgG spjälkat av trypsin (IgG TD) detekterades 26 glykopepeptider med UHPLC-MS genom att använda olika gradienter och mobilfaser. READ MORE

3. 2. Impact of PFAS exposure on the fecal metabolome

   University essay from Örebro universitet/Institutionen för naturvetenskap och teknik

   Author : Lisa Johansson; [2022]
   Keywords : Gut metabolites; LC-qTOF-MS; PFAS;

   Abstract : The human gut microbiota plays a crucial role in human health and therefore imbalances in gut microbiota functioning can lead to the development of metabolic disorders. Short-chain fatty acids (SCFA), tricarboxylic acid (TCA) cycle metabolites, bile acids (BA) and other metabolites are the essential compounds for the metabolome. READ MORE

4. 3. Impact of polychlorinated biphenyl- and organochlorine pesticide exposure on faecal metabolome

   University essay from Örebro universitet/Institutionen för naturvetenskap och teknik

   Author : Maja Näsman; [2022]
   Keywords : Gut microbiota; polychlorinated biphenyls; organochlorine pesticides; tricarboxylic acid cycle; bile acids; LC-qTOF-MS.;

   Abstract : The gut microbiota plays a major part in maintaining the health of a human host. Countless of crucial functions in the body, including immune responses, cell signaling and energy metabolism to name a few, are conducted by the gut microbiota and its metabolites. READ MORE

5. 4. Optimizing purification of oligonucleotides with reversed phase trityl-on solid phase extraction

   University essay from Örebro universitet/Institutionen för naturvetenskap och teknik

   Author : Ninorta Bartuma; [2019]
   Keywords : Oligonucleotides; purification; trityl-on; SPE; AIE-HPLC;

   Abstract : Oligonucleotides are synthetic strings of DNA or RNA used mostly for biochemical analysis and diagnostics. For them to be useful in these fields, a purity over 90% is most often required. However, when synthesizing these sequences, many “failures” (shorter sequences) are made in the step-wise process. READ MORE

6. 5. Development of separation method for analysis of oligonucleotides using LC-UV/MS

   University essay from Uppsala universitet/Analytisk farmaceutisk kemi

   Author : Björs Ida; [2018]
   Keywords : Oligonucleotides; UPLC; liquid chromatography; time of flight; mass spectrometry; reversed-phase weak anion exchange; AcclaimTM Mixed Mode WAX-1; DNAPacTM RP; ACQUITY UPLC BEH C18;

   Abstract : Introduction Oligonucleotides are short nucleic acid chains, usually 19-27mer long. They bind to their corresponding chain, making a specific inhibition possible. In pharmaceuticals, this can be used to inhibit the expression of a gene or protein of interest. READ MORE