Essays about: "live imaging"

Showing result 1 - 5 of 35 essays containing the words live imaging.

  1. 1. Cell Identification from Microscopy Images using Deep Learning on Automatically Labeled Data

    University essay from Lunds universitet/Institutionen för elektro- och informationsteknik

    Author : Fredrik Salomon-Sörensen; [2023]
    Keywords : Deep Learning; Cell Identification; Image Segmentation; Nuclei Segmentation; Convolutional Neural Networks; UNet; Image Analysis; Microscopy; Noisy Labels; Phase-Contrast; Automatic Labeling; Technology and Engineering;

    Abstract : In biology, cell counting provides a fundamental metric for live-cell experiments. Unfortunately, most researchers are constrained to using tedious and invasive methods for counting cells. Automatic identification of cells in microscopy images would therefore be a valuable tool for such researchers. READ MORE

  3. 2. Non-canonical amino acid incorporation as a strategy for labeling membrane bound Na+/K+-ATPase for fluorescence microscopy imaging

    University essay from KTH/Proteinvetenskap

    Author : Adam Johansson Holopainen; [2023]
    Keywords : ncAAs; fluorescence microscopy; amber suppression; nonsense suppression; Na K -ATPase; protein labeling; click chemistry; genetic code expansion; onaturliga aminosyror; fluorescensmikroskopi; Na K -ATPas; proteinmärkning; klickkemi; genetisk kodexpansion;

    Abstract : Natrium-kaliumpumpen spelar en väsentlig roll i en rad fysiologiska funktioner då den upprätthåller den elektrokemiska gradienten över cellmembranet. Ytterligare så är störningar i dess funktion associerade med flera neurologiska sjukdomar. READ MORE

  4. 3. Single-molecule Imaging of the Cell Division Ring in Escherichia coli Using the ALFA-tag

    University essay from KTH/Tillämpad fysik

    Author : Emma Westlund; [2023]
    Keywords : ALFA-tag; Escherichia coli; Divisome protein FtsZ; mEos3.2; Nanobodies; Nanotags; PALM; Plasmid transformation; sfGFP; Single-molecule Imaging; ALFA-tag; Escherichia coli; Divisomprotein FtsZ; mEos3.2; Nanokroppar; Nanotaggar; PALM; Plasmidtransformation; sfGFP; Enmolekyl-mikroskopi;

    Abstract : The use of super-resolution (SR) microscopy is an important tool for understanding the inside mechanisms of bacterial cells. However, for SR imaging, the labelling of the proteins of interest is a great challenge as flourescent proteins (FPs) are often too big to be directly fused to the target protein and traditional immunolabelling with antibodies creates too long separation between the fluorophore and the target protein. READ MORE

  5. 4. Surface Guided Helical Tomotherapy for Total Marrow Irradiation

    University essay from Lunds universitet/Sjukhusfysikerutbildningen

    Author : Hedda Enocson; [2022]
    Keywords : Medicine and Health Sciences;

    Abstract : Background: Total marrow irradiation (TMI) with helical tomotherapy treatment (HT-TMI) can be used as conditioning regimen for leukemia patients prior to stem cell transplantation by radiating the skeletal tissue. To ensure adequate dose coverage a margin is applied to the clinical target volume (CTV), i.e. planning target volume (PTV) margin. READ MORE

  6. 5. Diffusion and Camera-Noise Modelling for Analysis of Single-Particle Tracking Movies

    University essay from Lunds universitet/Beräkningsbiologi och biologisk fysik - Genomgår omorganisation

    Author : Erik Clarkson; [2022]
    Keywords : reaction-diffusion; camera-noise modelling; single-particle tracking; Gillespie simulations; Physics and Astronomy;

    Abstract : Interactions between T-cells and other body cells is an essential part of the immune system. It involves the binding between surface receptors of the two cells. Specifically, T-cell receptors (TCRs) bind onto pMHC (peptide-loaded major histocompatibility complex) molecules on the partnering cell surface. READ MORE