Essays about: "live-cell imaging"

Showing result 1 - 5 of 9 essays containing the words live-cell imaging.

  1. 1. Cell Identification from Microscopy Images using Deep Learning on Automatically Labeled Data

    University essay from Lunds universitet/Institutionen för elektro- och informationsteknik

    Author : Fredrik Salomon-Sörensen; [2023]
    Keywords : Deep Learning; Cell Identification; Image Segmentation; Nuclei Segmentation; Convolutional Neural Networks; UNet; Image Analysis; Microscopy; Noisy Labels; Phase-Contrast; Automatic Labeling; Technology and Engineering;

    Abstract : In biology, cell counting provides a fundamental metric for live-cell experiments. Unfortunately, most researchers are constrained to using tedious and invasive methods for counting cells. Automatic identification of cells in microscopy images would therefore be a valuable tool for such researchers. READ MORE

  3. 2. Non-canonical amino acid incorporation as a strategy for labeling membrane bound Na+/K+-ATPase for fluorescence microscopy imaging

    University essay from KTH/Proteinvetenskap

    Author : Adam Johansson Holopainen; [2023]
    Keywords : ncAAs; fluorescence microscopy; amber suppression; nonsense suppression; Na K -ATPase; protein labeling; click chemistry; genetic code expansion; onaturliga aminosyror; fluorescensmikroskopi; Na K -ATPas; proteinmärkning; klickkemi; genetisk kodexpansion;

    Abstract : Natrium-kaliumpumpen spelar en väsentlig roll i en rad fysiologiska funktioner då den upprätthåller den elektrokemiska gradienten över cellmembranet. Ytterligare så är störningar i dess funktion associerade med flera neurologiska sjukdomar. READ MORE

  4. 3. Single-molecule Imaging of the Cell Division Ring in Escherichia coli Using the ALFA-tag

    University essay from KTH/Tillämpad fysik

    Author : Emma Westlund; [2023]
    Keywords : ALFA-tag; Escherichia coli; Divisome protein FtsZ; mEos3.2; Nanobodies; Nanotags; PALM; Plasmid transformation; sfGFP; Single-molecule Imaging; ALFA-tag; Escherichia coli; Divisomprotein FtsZ; mEos3.2; Nanokroppar; Nanotaggar; PALM; Plasmidtransformation; sfGFP; Enmolekyl-mikroskopi;

    Abstract : The use of super-resolution (SR) microscopy is an important tool for understanding the inside mechanisms of bacterial cells. However, for SR imaging, the labelling of the proteins of interest is a great challenge as flourescent proteins (FPs) are often too big to be directly fused to the target protein and traditional immunolabelling with antibodies creates too long separation between the fluorophore and the target protein. READ MORE

  5. 4. Different concentrations of GSK3 inhibitor fail to suppress interleukin-6 in stimulated THP-1 macrophage

    University essay from Högskolan i Skövde/Institutionen för biovetenskap

    Author : Batoul Shunnar; [2022]
    Keywords : ;

    Abstract : Inflammation is a defensive process that allows immune cells to be mobilized to help with infection removal and tissue regeneration. Inflammasomes are multiprotein oligomers in the cytoplasm and components of the innate immune system that have a role in inflammation. READ MORE

  6. 5. Evaluation of In-Silico Labeling for Live Cell Imaging

    University essay from Umeå universitet/Institutionen för fysik

    Author : Elsa Sörman Paulsson; [2021]
    Keywords : Deep learning; Machine Learning; Image analysis; CNN; cell; live cell imaging; supervised learning; AI; artificial intelligence; sartorius;

    Abstract : Today new drugs are tested on cell cultures in wells to minimize time, cost, andanimal testing. The cells are studied using microscopy in different ways and fluorescentprobes are used to study finer details than the light microscopy can observe.This is an invasive method, so instead of molecular analysis, imaging can be used. READ MORE