Essays about: "mild elution"

Found 2 essays containing the words mild elution.

  1. 1. Optimization of Calcium-Dependent Affinity Ligands for Protein Purification

    University essay from KTH/Proteinvetenskap

    Author : Linnea Öst; [2021]
    Keywords : Protein purification; Protein A; calcium-dependent; scFv; G-CSF; multimerization; mild elution; ZCa;

    Abstract : With an expanding life-science sector and growing production of recombinant proteins, the need for efficient downstream processing is increasing. Certain proteins are sensitive to the harsh conditions often used in protein purification, such as low pH, which can result in aggregation and denaturation. READ MORE

  2. 2. Calcium-dependent affinity ligands for protein purification

    University essay from KTH/Skolan för kemi, bioteknologi och hälsa (CBH)

    Author : Emma Larsson; [2020]
    Keywords : Purification; Affinity chromatography; Protein A; Z domain; Calcium-dependent; ZCa; Elution;

    Abstract : The rapid growth of the biopharmaceutical industry has led to increasing demands on the protein production process. An important aspect is the yield of functional protein, which can be greatly affected by the choice of downstream purification. READ MORE