Essays about: "nucleic acid detection"

Showing result 1 - 5 of 7 essays containing the words nucleic acid detection.

  1. 1. Signal amplification in a microfluidic immunoassay system via Binding Oligo Ladder Detection : Applying the Exazym® signal amplification to the Gyrolab® platform

    University essay from Uppsala universitet/Institutionen för farmaci

    Author : Daniel Wiman; [2023]
    Keywords : immunoassay; ultra-sensitive; signal amplification; Gyrolab; Exazym; high sensitivity; microfluidics; BOLD amplification; nucleic acid polymerisation;

    Abstract : Immunoassays are analytical methods that use the highly specific binding of antibodies in order to detect and quantify an analyte. The technique has become a staple in modern biopharmaceutical research and diagnostics, however the measurement of biomarkers like dysregulated cytokines require ultra-sensitive immunoassays that can detect molecules at sub pg/mL concentrations. READ MORE

  3. 2. Simplified Quality Control of Coating Efficiency for a Cancer Monitoring Assay

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Karl Andersson; Isa Beskow; Pinja Moshiri; Victoria Niemi; Elin Nygren; Stina Åström; [2022]
    Keywords : Quality control; nucleic acid detection; poly-A templates; cancer monitoring assay; BrdUTP; biotin; digoxigenin;

    Abstract : The aim of this report is to present a basis for development of an alternative quality control method for a part of the cancer monitoring technique DiviTum® by Biovica. The methods recommended are direct implementation of bromodeoxyuridine (BrdUTP), as well as biotin and digoxigenin systems. READ MORE

  4. 3. Development of separation method for analysis of oligonucleotides using LC-UV/MS

    University essay from Uppsala universitet/Analytisk farmaceutisk kemi

    Author : Björs Ida; [2018]
    Keywords : Oligonucleotides; UPLC; liquid chromatography; time of flight; mass spectrometry; reversed-phase weak anion exchange; AcclaimTM Mixed Mode WAX-1; DNAPacTM RP; ACQUITY UPLC BEH C18;

    Abstract : Introduction Oligonucleotides are short nucleic acid chains, usually 19-27mer long. They bind to their corresponding chain, making a specific inhibition possible. In pharmaceuticals, this can be used to inhibit the expression of a gene or protein of interest. READ MORE

  5. 4. Exploration of how UV disinfection affects the microbial community in drinking water

    University essay from Lunds universitet/Teknisk mikrobiologi

    Author : Sakarias Ekman; [2018]
    Keywords : heterotrophic plate count; qPCR; flow cytometry; drinking water; UV disinfection; bacterial community; applied microbiology; teknisk mikrobiologi; Technology and Engineering; Biology and Life Sciences;

    Abstract : UV irradiation is getting increasingly popular as a disinfection step in drinking water production. Ringsjöverket, a drinking water treatment plant in Sweden installed a full-scale UV aggregate in December 2016. However, the effect of UV irradiation on drinking water has mainly been tested on specific pathogens in labscale environment. READ MORE

  6. 5. Image Sensor System for Detection of Bacteria and Antibiotic Resistance

    University essay from KTH/Skolan för teknik och hälsa (STH)

    Author : Ejla Lillro; [2015]
    Keywords : CMOS; CCD; image sensor; fluorescence imaging; Lab-on-a-chip; digitalPCR; urinary tract infections; point-of-care;

    Abstract : Antibiotic resistance is now a recognized problem in global health. In attempts to find solutions to detect bacteria causing antibiotic resistance we turn to technological solutions that are miniaturized, portable and cheap. READ MORE