Essays about: "plasmid transfection"

Showing result 1 - 5 of 10 essays containing the words plasmid transfection.

  1. 1. Design and production of adeno-associated virus vectors for imaging mitochondrial networks in the brain

    University essay from Uppsala universitet/Institutionen för biologisk grundutbildning

    Author : Elnaz Samadian Zad; [2023]
    Keywords : adeno-associated virus; mitochondria; mitochondrial networks; imaging; fluorescent protein; synapsin promoter; human embryonic kidney cells; organoids;

    Abstract : Mitochondria are dynamic organelles that function in a complex interconnected network within the cell. Neurons are sensitive and highly energy demanding cells in the brain which require a functioning mitochondrial network that is able to provide ATP and modulate calcium. READ MORE

  3. 2. The use of Gibson Assembly for DNA cloning

    University essay from KTH/Tillämpad fysik

    Author : Samuel Johansson; [2022]
    Keywords : Plasmid; Primer; Cloning; PCR; Gibson-Assembly; Transfection; Fluorescent protein; Fluorescent imaging; mCherry; EGFP; Zona-Occludens 1; Occludin; alphaTubulin; Cx-43-7; Plasmid; Primer; Kloning; PCR; Gibson-Assembly; Transfektion; Fluorescerande protein; Fluorescerande avbildning; mCherry; EGFP; Zona-Occludens 1; Occludin; alphaTubulin; Cx-43-7;

    Abstract : This thesis report revolved around the cloning process of plasmids. Attempts of cloning the red fluorescent protein mCherry, and the green fluorescent protein EGFP from various plasmids, into other plasmids containing different cell-junction/cytoskeleton plasmids were made. READ MORE

  4. 3. Hypoxia-induced expression of HIF-1a and GPR30, in relation to survival, proliferation and apoptosis in MCF-7 and SkBr3 breast cancer cells.

    University essay from Lunds universitet/Läkarutbildningen

    Author : Hanna Chahin; Dianna Jazera; [2021]
    Keywords : hypoxia; breast cancer; GPR30 estrogen receptor; Medicine and Health Sciences;

    Abstract : Introduction: Hypoxia that accompanies tumor growth induces the expression of hypoxiainducible factor a (HIF-1a), in turn mediating the expression of other genes, including G protein-coupled receptor 30 (GPR30), to promote adaption to the hypoxic microenvironment and enable cell proliferation and survival. Nonetheless, this correlation has not been causally explained. READ MORE

  5. 4. Increased expression of therapeutic proteins by identification of 3'-UTRs from high expressing genes in CHO cells

    University essay from Linköpings universitet/Institutionen för fysik, kemi och biologi

    Author : Alexander Westlund; [2019]
    Keywords : Mammalian cell; CHO; E. coli; protein expression; plasmid; transformation; Fc-fusion protein; flow cytometry; FACS; transfection; mRNA; UTR; gene engineering;

    Abstract : Therapeutic proteins, a.k.a. biopharmaceuticals, are most commonly produced in expression systems derived from Chinese Hamstery Ovary (CHO) cells, thanks to great capacity of post-translational modifications like secretation, folding and glycosylation. READ MORE

  6. 5. Effect of transfection reagents on plasmid promoters

    University essay from Uppsala universitet/Biokemi

    Author : Yi Lu; [2018]
    Keywords : ;

    Abstract : .... READ MORE